幽门螺杆菌慢性感染诱导胃上皮细胞产生凋亡耐受  

Chronic Helicobacter pylori infection induces apoptosis-resistance in gastric epithelial cells

作　　者：何兴祥[1] 胡品津[1] Benjamin Chun Yu Wong~2 Shiu Kum~2 Lam Harry Hua Xiang Xia~2 

机构地区：[1]广州中山医科大学第一附属医院消化科,510080 [2]Departments of Medicine, The University of Hong Kong,Queen MaryHospital,Hong Kong

出　　处：《现代消化及介入诊疗》2002年第3期34-38,共5页Modern Interventional Diagnosis and Treatment in Gastroenterology

摘　　要：目的建立幽门螺杆菌(H.pylori)慢性感染胃上皮细胞模型,并分析H.pylori慢性感染与胃上皮细胞凋亡的关系.方法首先用H.pylori SSI与人非肿瘤性胃上皮细胞株GES-1共培养16周,建立GES-1模型细胞,然后采用流式细胞术分析这种模型细胞对H.pylori和其它肠道细菌以及抗肿瘤药物凋亡诱导剂的应答.结果成功获得了H.pylori慢性感染胃上皮细胞模型(GES-1模型细胞).这种模型细胞不仅对H.pylori诱导的凋亡耐受,而且对其他肠道细菌和一些凋亡诱导剂也是耐受的。结论幽门螺杆菌慢性感染诱导胃上皮细胞产生凋亡耐受,由此可能增加了胃上皮细胞癌变的危险性.Objective To establish a cell model by chronically infecting human gastric epithellial cells with H.pylori, and to determine the effect of chronic H.pylori infection on apoptosis of gastric epithelial cells.Methods Human non tumor gastric epithelial cells GES-l were cocultured with a H.pylori strain SSI for 16 weeks, in order to obtain GES-l 'model' cells.Biological characteristics of the model cells including growth, adherence and clone formation were determined. Apoptosis of the model cells in response to apoptosis inducers such as H.pylori and other enterobacteria and agents commonly used in the obtain GESl model cells.Biological characteristics of the 'model' cells including growth, adherence and clone formation were determined. Apoptosis of the ' model' cells in response to apoptosis inducers such as H.pylori and other enterobacteria and agents commonly used in the chemotherapy of gastric cancer were measured by flow cytometry. Results A cell model of human gastric epithelial cells with chronic H.pylori infection (GES-l model cells) was successfully established.Apoptosiswas dramatically decreased in the 'model' cells with and without stimulation by H.pylori and other enterobacteria and some chemotherapeutic agents. Conclusion Chronic H.pylori infection induces apoptosis resistance in gastric epithelial cells, which may increase the risk of the development of gastric cancer.

关 键 词：幽门螺杆菌慢性感染 诱导 胃上皮细胞 产生 凋亡 耐受 

分 类 号：R573.3[医药卫生—消化系统]