药物干预对6-羟基多巴胺处理大鼠的纹状体细胞外液多巴胺的影响  

Effects of medications on dopamine of rat corpus striatum treated with 6-OHDA

作　　者：徐耀[1] 包仕尧[1] 罗蔚锋[1] 张志琳[1] 

机构地区：[1]苏州大学附属第二医院神经内科,215004

出　　处：《江苏医药》2003年第5期328-330,共3页Jiangsu Medical Journal

摘　　要：目的　研究古拉定、川芎嗪、丹参对 6 羟基多巴胺 ( 6 OHDA)处理后大鼠的纹状体细胞外液多巴胺 (DA)及其代谢产物的影响。方法　运用 6 OHDA损毁大鼠黑质 ,设立正常对照组、6 O HDA损毁组、损毁后古拉定治疗组、川芎嗪治疗组和丹参治疗组。损毁后 8周用微透析法采集各组大鼠纹状体细胞外液 ,用高效液相色谱 电化学法 (HPLC ECD)检测DA、DOPAC、HVA。结果　发现四组损伤组的DA及其代谢产物含量明显低于正常对照组 (P <0 .0 0 1) ,而三组治疗组与非治疗组组间比较无统计学差异 (P >0 .0 5 )。结论　三种药物不能改变 6Objective To study the effect of GSH,Ligustrazine,Salvia miltiorrhiza on dopamine and its metabolites of corpus striatum in rats treated with 6 OHDA.Methods Male SD rats were divided into five groups at random:control rats,6 OHDA lesioned rats without treatment,and 6 OHDA lesioned rats treated with GSH,Ligustrazine or Salvia miltiorrhiza.6 OHDA(12μg/μl)was injected into right substantia nigra pars compacta(SNpc )by two sites of the rat at day 0.In treatment groups,GSH(12mg·kg -1 ·d -1 ),Ligustrazine(50mg/kg/d)or Salvia miltiorrhiza(2 5ml·kg -1 ·d -1 )was i.p.injected every day from day 0 to day 14 to each rat.In day 60,extracellular dopamine (DA) and its metabolites(DOPAC,HVA)in the striatum of all groups were collected by microdialysis and measured by high performance liquid chromatography with electrochemical detection(HPLC ECD).Results In controls,extracellular DA and its metabolites DOPAC,HVA were significantly higher than those of other groups in right sides( P< 0 001).But there are no differences between rats with 6 OHDA and rats with Ligustrazine,GSH or Salvia miltiorrhiza.Conclusion Three medicines could not alter DA in extracellular of corpus striatum in rats dealing with 6 OHDA

关 键 词：6-羟基多巴胺 帕金森病 多巴胺 川芎嗪 古拉定 丹参 脑内微透析技术 高效液相色谱-电化学法 

分 类 号：R742.5[医药卫生—神经病学与精神病学]