RASSF1基因不同转录本在肺癌组织中的转录表达及临床意义  

Expression of different RASSF1 transcripts and its clinical significance in lung carcinoma

作　　者：邵康[1] 赫捷[1] 程邦昌[2] 肖智雄[3] 张德超[1] 黄微[1] 张翠艳[1] 周芳[1] 熊美华[1] 唐槐静[1] 石素胜[1] 姆巴东[2] 

机构地区：[1]中国医学科学院中国协和医科大学肿瘤研究所肿瘤医院胸外科实验室,北京研究生100021 [2]武汉大学人民医院胸心外科 [3]美国波士顿大学医学院生化系与医学系

出　　处：《中华肿瘤杂志》2003年第2期149-152,共4页Chinese Journal of Oncology

基　　金：国家科技攻关计划项目 ( 2 0 0 2BA711A0 6)

摘　　要：目的　探讨RASSF1基因 3种不同转录本在肺癌中的表达情况及其临床意义。方法采用RT PCR方法检测 5 1例肺癌癌组织及相应癌旁正常组织中RASSF1A、RASSF1B及RASSF1CmRNA的表达情况。结果　 (1) 3种不同转录本在癌旁正常肺组织中均表达 ;在癌组织中 ,RASSF1A及RASSF1B存在较高的表达缺失 ,其表达缺失率分别为 5 4 .9% (2 8/ 5 1)和 37.3% (19/ 5 1) ,RASSF1C则全部表达。 (2 )RASSF1A表达与肺癌淋巴结转移及病理分期有关 ,淋巴结转移者及晚期病例均存在较高的RASSF1A表达缺失 (P <0 .0 5 )。 (3)RASSF1B和RASSF1C表达与肺癌分期、组织学类型、分化程度及吸烟指数等均无显著相关性 (P >0 .0 5 )。结论　RASSF1基因 3种不同转录本在肺癌中的表达存在明显差异 ,其中转录本A与肺癌淋巴结转移及病理分期相关 ,是一新型肺癌抑癌基因。Objective To evaluate the expression of three different RASSF1 transcripts and its clinical significance in lung carcinomas. Methods The mRNA expression of RASSF1A, RASSF1B and RASSF1C was detected by RT PCR in 51 human lung cancer tissues and 51 matched normal tissues. Results 1. The mRNA expression of three RASSF1 transcripts was detectable in all non cancer tissues. However, high rate of expression loss of RASSF1A and RASSF1B existed in lung cancer tissues, which was 53.2%(2851) and 37.3%(19/51), respectively. RASSF1C was expressed in all of the tumor tissues. 2. Loss or abnormal down regulation of RASSF1A was positively related with lymph node metastasis and TNM stage ( P <0.05) and 3. RASSF1B and RASSF1C mRNA expression was not correlated with TNM stage, histological type, differentiation grade or smoking index. Conclusion There is a significant expression difference among the three RASSF1 transcripts in lung carcinoma. RASSF1A, closely associated with lymph metastasis and TNM stage of lung carcinoma, should be a new tumor suppressor gene.

关 键 词：肺癌 RASSF1基因 基因转录 转录本A 淋巴结转移 病理分期 

分 类 号：R734.2[医药卫生—肿瘤]