hRad17 mRNA在胃癌中表达的意义  

Significance of hRad17 mRNA expression in human gastric cancer

作　　者：黄宝俊[1] 赵雨杰[2] 徐惠绵[1] 张玉魁[2] 王绍成[2] 徐莹莹[1] 

机构地区：[1]中国医科大学附属第一医院肿瘤外科,沈阳110001 [2]中国医科大学生物芯片中心

出　　处：《中华肿瘤杂志》2003年第2期160-162,共3页Chinese Journal of Oncology

基　　金：辽宁省科技厅十五攻关基金资助项目 ( 2 0 0 12 2 5 0 0 2 3 ) ;辽宁省自然科学基金资助项目 ( 2 0 0 110 10 0 1)

摘　　要：目的　研究hRad17mRNA表达与胃癌临床病理因素及淋巴结转移的关系 ,探讨其术前预测淋巴结转移危险度及预后的意义。方法　采用RT PCR、聚丙烯酰胺凝胶电泳银染色法 ,检测 5 2例胃原发癌、正常黏膜、淋巴结转移癌及 5例胃良性病变和SGC790 1胃癌细胞系中hRad17mRNA的表达。结果　hRad17mRNA在上述组织细胞中均有表达 ,胃原发癌和淋巴结转移癌明显高于正常黏膜(P =0 .0 14 ,P =0 .0 0 1)。胃原发癌hRad17mRNA表达与转移淋巴结数量具有相关性 ,转移淋巴结数量 >15者高于 <15者 (P =0 .0 2 ) ;弥漫性生长者高于团块状生长者 (P =0 .0 4 )。结论　聚丙烯酰胺凝胶电泳银染色明显提高了检测的敏感性 ;胃原发癌hRad17mRNA过度表达的患者淋巴结转移范围及程度相对较重 ,癌细胞侵袭力较强 ,易行扩大根治术 ;术前RT PCR检测胃原发癌hRad17mRNA表达有助于确定治疗方案及判断预后。Objective To study the relationship between hRad17 mRNA expression and clinicopathologic factors and lymph node metastasis of gastric cancer, and to assess the significance of predicting the extent of lymph node metastasis and prognosis. Methods hRad17 mRNA expression was examined in matched primary lesions, normal gastric mucosa and lymph node metastatic lesions among 52 gastric cancer patients by reverse transcription polymerase chain reaction(RT PCR), polyacrylamide gel electrophoresis(PAGE) and silver stain with the relation between hRad17 mRNA expression and clinicopathologic factors analyzed. At the same time, hRad17 mRNA expressions in 5 gastric benign lesions and SGC7901 gastric carcinoma cell lines were also examined. Results The primary tumor samples(88.4% positive)showed a significantly higher level of hRad17 expression compared with matched normal tissue(76.9% positive) ( P =0.014), so did the lymph node metastatic samples(94.2% positive)( P =0.001). The hRad17 mRNA expression showed a low level in benign lesions, but very high in SGC7901 cell line. The hRad17 mRNA expression showed a higher level in patients with the number of lymph node metastasis above 15 than below 15( P =0.02), so did the diffused growth than the mass like growth ( P =0.04). Conclusion The method of PAGE and silver stain can improve the sensitivity of RT PCR. The degree of lymph node metastasis and invasiveness of carcinoma cells are more serious in cases with hRad17 mRNA overexpression, and extensive lymph node dissection should be carried out for these patients. Examination of hRad17 expression by RT PCR before surgery is indicated to arrive at an optimum treatment scheme and to estimate the prognosis.

关 键 词：胃癌 hRadl7 MRNA表达 淋巴结转移 RT-PCR检测 聚丙烯酰胺凝胶电泳银染色 

分 类 号：R735.2[医药卫生—肿瘤]