茶叶中单宁的荧光动力学分析法研究  

Study on the Kinetic Fluorimetric Determination of Tannins in Tea

作　　者：冯素玲[1] 汤俊明[1] 樊静[1] 

机构地区：[1]河南师范大学化学与环境科学学院

出　　处：《光谱学与光谱分析》2003年第2期322-324,共3页Spectroscopy and Spectral Analysis

基　　金：河南省教委自然科学基金(No.1999150030);河南省高等学校环境科学与工程重点学科开放实验室资助项目

摘　　要：本文根据在Britton-Robinson缓冲溶液中单宁对Cu(Ⅱ)催化过氧化氢氧化吡咯红Y具有活化作用,使体系荧光狡灭,建立了一个高灵敏度荧光动力学测定痕量单宁的新方法。详细研究了体系的最佳实验条件,采用固定时间法测定体系的荧光强度。该方法的线性范围为0.06～0.96mg·L^(-1),检出限为0.032mg·L^(-1)。对含量为0.32mg·L^(-1)的单宁标准溶液进行11次平行测定,其相对标准偏差为2.3％。将方法用于茶叶中单宁含量的测定,并与Folin-Ciocalteu光度法作了对照,结果满意。A simple and highly sensitive kinetic fluorimetric method is proposed for the determination of trace tannins, based on the activation of tannins on the oxidation of pyronine Y by hydrogen peroxide catalyzed by Cu(U)ion. The effects of some experimental conditions were investigated and discussed in detail. The fixed reaction time procedure was used to determine the fluorescence intensity of the system. The calibration curve of tannin was linear in the range of 0.06-0.96 mg.L-1 and the detection limit for tannin was 0.032 mg.L-1. The relative standard deviation for the measurement of 0.32 mg.L-1 tannin (n = 11) was 2.3%. The proposed method has been successfully applied to the determination of tannins in tea. The results obtained were compared with those provided by the Folin-Ciocalteu method.

关 键 词：茶叶 单宁 荧光动力学分析法 吡咯红Y 

分 类 号：TS272.7[农业科学—茶叶生产加工]