血啉甲醚的时间分辨荧光光谱研究  

Time-resolved Fluorescence Studies of Hematoporphyrin Monomethyl Ether for Photodynamic Diagnosis

作　　者：李步洪[1] 陆祖康[1] 谢树森[2] 

机构地区：[1]浙江大学国家光学仪器工程技术研究中心,浙江杭州310027 [2]福建师范大学激光研究所生物医学光学实验室,福建福州350007

出　　处：《光谱学与光谱分析》2003年第2期331-333,共3页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金(60178022);福建省自然科学基金(2002F008)

摘　　要：实验研究了用于肿瘤光动力学诊断的第二代新型光敏剂血啉甲醚(HMME)分别在不同血型人血清和生理盐水环境中的时间分辨光谱,同时还研究了在不同荧光发射峰处的荧光寿命以及光敏剂浓度对荧光寿命的影响。结果表明:HMME在625和690nm处的荧光寿命基本相同,无显著差异。HMME在生理盐水和人血清环境中的荧光寿命具有显著差异,分别为14.6和16.6ns,同时实验结果还表明光敏剂浓度对荧光寿命没有影响。结论对于开展肿瘤的时间分辨药物荧光光谱诊断与成像技术具有重要的指导意义。Time-resolved fluorescence spectroscopic measurements of second-generation new photosensitizer of hematoporphyrin monomethyl ether (HMME) were carried out in the physiological saline and different human serums by picosecond fluorescence lifetime spectrometer, respectively. The objective was to assess the fluorescence lifetime of HMME as a new enhanced contrast parameter between normal and malignant tissues for the photodynamic diagnosis of various cancers. The influence of emission wavelength and photosensitizer concentration, on the lifetime of HMME was also presented. No significant change in fluorescence decay was found between emission wavelength 625 and 690 rim. The lifetimes of HMME diluted in the physiological saline and human serum were about 14.6 and 16.6 ns, respectively. Experimental results also indicate a quite stable behavior with a decay time of 16.6 ns when HMME was diluted with different concentration. Time-resolved fluorescence spectrum of HMME can serve as a characteristic marker for optical biopsy of the malignant tissue because of the useful property of selective retention in malignant tumors after intravenous injection. Furthermore, the results in this study will contribute to the improvement of nanosecond fluorescence lifetime imaging method.

关 键 词：血啉甲醚 时间分辨荧光光谱 肿瘤 光动力学诊断 光敏剂 

分 类 号：R730.43[医药卫生—肿瘤]