禽I型副粘病毒f基因克隆及序列分析  

Cloning and Sequencing of f gene of Avian paramyxovirus TypeⅠIsolates

作　　者：宋建领[1] 赵文华[1] 杨斌[1] 张永仙[1] 王金萍[1] 信爱国[1] 朱建波[1] 丛佩清[1] 

机构地区：[1]农业部热带亚热带动物病毒学重点开放实验室,云南昆明650224

出　　处：《中国病毒学》2003年第2期141-145,共5页Virologica Sinica

摘　　要：用RT-PCR一步法对云南省不同禽类(鸡、鸽子)3株禽I型副粘病毒F基因进行扩增和克隆,并对其f基因片段核苷酸序列进行分析,结果表明,云南省禽I型副粘病毒各毒株同源性为88.1%～94.9%,与疫苗株LaSota和强毒株F48E9的同源性为85.6%。所分离两株新城疫病毒在F蛋白裂解位点区(112～117aa)的氨基酸序列与强毒株在这一区域的序列完全相同,表明为强毒株。鸽I型副粘病毒F蛋白裂解位点区的氨基酸序列与PPMV ZQ98-1株在这一区域的序列完全相同,揭示为中强毒株。以1 662bp核苷酸绘制系统发育树,表明云南地方新城疫病毒属于基因Ⅶ型,鸽I型副粘病毒属于基因Ⅵ型。The f gene was amplified with one-step RT-PCR, cloned and sequenced from three virus isolates (Avian paramyxovirus typeⅠ), which were isolated from different avian (chicken and pigeon) inYunnan province. The result of the nucleotide sequence analysis indicated that the homology between the three strains studied was 88.1%~94.9%; while the studied strains with reference strains LaSota and F48E9 only showed 85.6% homology. The study on the cleavage site amino acid sequence deduced from the nucleotide sequence (residues 112~117) showed that the two NDV strains matched to all NDV velogenic strains and the Pigeon paramyxovirus (PPMV)Ⅰstrain matched to PPMV ZQ98-1 strain (one mesogenic strain), so the former were velogenic strains and the later was mesogenic strain. Phylogenetic tree constructed with 16 reference strains of 1 662nt (ORF region) of the f gene showed that the two NDV strains should belongs to genotype Ⅶ and the PPMV strain belongs to genotype Ⅵ.

关 键 词：禽Ⅰ型副粘病毒 F基因 基因克隆 序列分析 RT-PCR一步法 同源性 系统发育 

分 类 号：S852.65[农业科学—基础兽医学]