大鼠局灶性脑缺血再灌注碱性成纤维细胞生长因子蛋白表达的研究  

The Studies of Basic Fibroblast Growth Factor Proteinsafter Focal Cerebral Ischemic and Reperfusion in Rats

作　　者：张睿[1] 徐洋[1] 房学迅[1] 包雪鹦[1] 

机构地区：[1]吉林大学第一医院,吉林长春130021

出　　处：《北华大学学报（自然科学版）》2003年第2期138-141,共4页Journal of Beihua University（Natural Science）

基　　金：国家自然科学基金(200230270332号)

摘　　要：目的检测大鼠局灶性脑缺血再灌注后中心区、半影区bFGF的蛋白表达动态变化及意义.方法采用线栓法制作大鼠局灶性脑缺血再灌注模型,采用神经病学评分,TTC染色,光镜检测对模型予以评价,并用免疫组化法观察缺血3 h再灌注3,6,24,72h缺血中心区、半影区bFGF表达的动态变化,并观察相应时间点、相应区域病理组织学变化.结果在正常组及假手术组bFGF呈弱阳性表达.缺血3 h及缺血3 h再灌注3,6 h中心区、半影区表达明显增强.与假手术组比较,差异显著(P＜0.05).缺血3 h再灌注24,72 h缺血中心区bFGF表达下降,但仍较正常对照组高,此时半影区表达呈持续升高趋势,24 h达高峰,72 h有所下降.而相应病理变化为:缺血3 h可见轻重不等的神经细胞变性坏死,缺血3 h再灌注中心区及半影区随缺血再灌注时间延长,病理组织学变化逐渐加重.结论正常脑组织中bFGF呈弱阳性表达,缺血中心区及半影区随缺血再灌注2 d内随时间延长表达增强,与神经细胞缺血改变加重呈正相关.提示bFGF对脑缺血损伤后的神经细胞有保护和修复作用.Objective To study the effects of bFGF expression after focal cerebral ischemic and reperfusion in rats. Methods Duplicating a reperfusion mouse model with focal cerebral ischemia in rats by filaments occlusion and neurological scale, TTC stain, pathohistology method to evaluate the model. On the basis of this, using immunohistochemistry method to observe the expression of bFGF during reperfusion after focal cerebral ischemia in rats. Results The expression of bFGF is slightly positive in control and sham-operated group. The expression of bFGF increases obviously in ischemic core and penumbra after 3 h reperfusion after 3 h of ischemia. With the elongation of the time of reperfusion after ischemia the expression of bFGF decreases gradually. While the expression of bFGF increases gradually, it reaches its peak after 24 h, it decreases after 72 h of reperfusion. Conclusion The expression of bFGF is slightly positive in control and sham-operated group. With the elongation of the time of reperfusion after ischemia, the expression of bFGF increases in ischemic core and penumbra in the two days. The nerve cells in ischemic core become necrotic with the elongation of reperfusion time after ischemia and the ischemia of neurons in penumbra become more apparent. Indicating that bFGF can protect and repair the nerve cells that have damaged after focal erebral ischemic and reperfusion in rats.

关 键 词：碱性成纤维细胞生长因子 凋亡 大鼠 局灶性脑缺血再灌注损伤 蛋白表达 神经细胞 保护作用 

分 类 号：R743.31[医药卫生—神经病学与精神病学] R－332[医药卫生—临床医学]