由ACC合成酶反义基因转化所得枣树其RNA表达量的测定  

Quantitation of Anti-ACC Synthase Gene RNA Expression in Transgenic Zixyphus jujuba by Real- time Reverse-transcription PCR Assay

作　　者：何业华[1] 林顺权[1] 林良斌[2] 熊兴华[3] 今石浩正 

机构地区：[1]华南农业大学园艺生物技术研究所,广东广州510642 [2]云南农业大学烟草学院,云南昆明650201 [3]湖南农业大学作物基因工程湖南省重点实验室,湖南长沙410128 [4]日本神户大学基因研究中心

出　　处：《湖南农业大学学报（自然科学版）》2003年第2期112-114,共3页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：国家自然科学基金资助项目(30070365)

摘　　要：利用real timeRT PCR对转基因枣树中的anti ACSG的RNA表达量进行了定量测定.样品扩增产物荧光量随着扩增循环数的增加而呈现"S"形增加;经熔解曲线分析,其扩增产物中未检出非特异性产物和引物二聚体;扩增产物的CT值和模板cDNA起始浓度两者之间呈线性相关(R2=0.9929);转化体中anti ACSGRNA表达量约为4500～10300拷贝/g组织.The quantitative determination of antiACC synthase gene RNA expression in transgenic Zixyphus jujuba plant is carradout by real time reversetranscription PCR. Results reveal that the amplification products fluorescence values of samples in crease in the form of'S'with the increase of cycle numbers. A melting curve analysis shows that, the quantity of unspecific PCR products have witch fewer primer dimers than other amplification products. Results show that there exists a linear correlation(R2=0.992 9) between the threshold cycle values of amplification products and the starting concentration of template cDNA. In the transformants, the expression quantitation of antiACC synthase gene is 4 50010 300 copies/g tissue.

关 键 词：REAL-TIMEPCR RT-PCR 反义-ACSG RNA 定量分析 枣树 ACC合成酶 反义基因 

分 类 号：S665.1[农业科学—果树学]