Real time observation of the photocleavage of single DNA molecules  

作　　者：LI Bin , HU Jun , WANG Ying, WU Shiying, HUANG Yibo & LI Minqian Shanghai Institute of Nuclear Research,Chinese Academy of Sciences, Shanghai 201800,China 

出　　处：《Chinese Science Bulletin》2003年第7期673-675,共3页

基　　金：supposed by the National Natural Science Foundation of China(Grant No.19890385);the fund from the Chinese Academy of Sciences(Grant Nos.STZ-00-07,KJCX-06 and KSCXI-06);the Science and Technology Commission of Shanghai(Grant No.00XD1444029).

摘　　要：A method for real time observation of photo-cleavage of stretched λDNA at single molecular level by a fluorescent microscope coupled with CCD is developed. DNA molecules stained with YOYO-1 are stretched by the mo-lecular combing technique and fixed on a modified slide. Then the process of photocleavage and relaxation of DNA under radiation of blue light is observed. We speculate that the conformation change of stretched DNA and the effect of water are likely to facilitate the effect of YOYO photocleav-age DNA molecules. The photocleavage effect of YOYO for stretched DNA may be useful to study DNA elasticity, cancer research as well as the interaction between DNA and dyes.A method for real time observation of photo-cleavage of stretched λDNA at single molecular level by a fluorescent microscope coupled with CCD is developed. DNA molecules stained with YOYO-1 are stretched by the mo-lecular combing technique and fixed on a modified slide. Then the process of photocleavage and relaxation of DNA under radiation of blue light is observed. We speculate that the conformation change of stretched DNA and the effect of water are likely to facilitate the effect of YOYO photocleav-age DNA molecules. The photocleavage effect of YOYO for stretched DNA may be useful to study DNA elasticity, cancer research as well as the interaction between DNA and dyes.

关 键 词：单个DNA分子 光致断裂 原子力显微镜 荧光显微镜 实时观察 生物大分子 

分 类 号：R730.2[医药卫生—肿瘤]