死胎胸腺组织中HBVDNA表达的初步探讨  

The Primary Study on the Expression of HBVDNA in the Thymus Tissues of the Died Fetus that the Puerperal Delivery with Hepatitis Virus B

作　　者：赵伟[1] 刘伟[1] 罗婵[1] 刘兰侠[1] 王兰[1] 

机构地区：[1]东南大学医学院附属南京市第二医院,江苏南京210008

出　　处：《中国实验诊断学》2003年第2期107-108,共2页Chinese Journal of Laboratory Diagnosis

基　　金：铁道部 2 0 0 0年自然科技资金资助 (J2 0 0 0Z0 95 )

摘　　要：目的　观察死胎胸腺组织中HBVDNA表达的情况 ,探讨通过产妇传播到死胎胸腺组织中的HBV是否存在复制。方法　采集 4 0例乙型肝炎产妇产下的死胎 ,常规尸检 ,取胸腺组织。采用原位分子杂交法 ,检测其中的HBVD NA ;回访产妇产前静脉血HBVM的检测结果。结果　死胎胸腺组织中HBVDNA的检出率为 2 7/40 (6 8% )例。产妇产前静脉血HBV呈高、低和无复制状态时 ,胸腺组织中HBVDNA的检出率分别为 2 /4(5 0 % )例、11/2 0 (5 5 % )例和 14 /16(88% )例 ;高、低、无复制者之间相互比较 ,差异不显著 (P >0 .0 5 )。HBVDNA颗粒分布在胸腺细胞浆内、细胞间和毛细血管内 ,少数分布在胸腺细胞核上。结论　死胎胸腺组织中有HBV复制 ;死胎胸腺组织中HBVDNA表达与产妇血清中HBV复制状态无关。Objective To observe the expression of HBVDNA and if there were copy of HBVM in the thymus tissues of the died fetus that puerperal infection with hepatitis virus B. Methods 40 cases such died fetus were collected and were made the autopsy for thymus tissues and detected HBVDNA with in situ molecule hybridization technique. Investigated the detection on HBVM in the mother veins before delivery. Results For the thymus tissues, there were 68%(27/40)cases detected out HBVDNA. When the HBV was high 、low and without replication status, the detecting rates were 50%(2/4),55%(11/20)cases and 88%(14/16)respectly . For the rate of HBVDNA , it was not statistically significant for the differences among the high and low,without replication status ( P >0.05). HBVDNA were mainly in the thymus cytoplasm,capillary vessel and among the thymus cells , few in the nuclei. Conclusion There were HBV replication in the thymus tissues of the died fetus. It was not related with the HBV replication status in the pregnant woman veins for expression of HBVDNA in the thymus tissues of the died fetus.

关 键 词：死胎 胸腺组织 HBVDNA 表达 原位分子杂交 乙型肝炎病毒脱氧核糖核酸 

分 类 号：R361[医药卫生—病理学]