体外培养的角膜缘上皮细胞生物学特性  

Observation on Biological Character of Cultivated Cornea Limbal Epithelial Cells

作　　者：刘兴华[1] 朱美玲[1] 陈逖[1] 王明丽[2] 刘伦[1] 

机构地区：[1]安徽医科大学第一附属医院,安徽合肥230022 [2]安徽医科大学微生物学教研室

出　　处：《中国医师杂志》2003年第4期450-452,共3页Journal of Chinese Physician

基　　金：安徽省教育委员会基金项目 (2 0 0 0jI1 0 3)

摘　　要：目的　观察体外培养的角膜缘上皮细胞生物学特性。方法　体外组织块培养兔角膜缘上皮细胞 ,倒置显微镜观察活细胞生长情况 ,透射电镜观察其超微结构 ,用流式细胞仪检测细胞的增殖能力 ,使用MTT方法绘制传代细胞的生长曲线。结果　角膜缘组织块培养第 6d培养的角膜缘上皮细胞形成单层 ,培养的细胞具有代谢旺盛和较高的增殖能力 ,传代的角膜缘上皮细胞在第4d达生长高峰。结论　体外培养的第 1、2代角膜缘上皮细胞既保持角膜上皮特性 ,又具有强的增殖能力。Objective To observe biological character iistcs of cultivated cornea limbal epithelial cells of rabbit.Methods Cultivated cornea limbal epithelial cells with tissue inoculation were observed by inverted microscope every two days; ultrastructure of cell was observated by electron microscope; Proliferative ability of cell was examined by flow cytometry; the cell growth curve was examined by monotetrazolium(MTT) colorimetry.Results Cultivated limbal cornea epithelial cells formed monolayer at six day,the cultivated conea limbal epithelial cells had high metabolism and proliferative ability, growth of cornea limbal epithelial cells reached climax after 4 days by passage culture. Conclusion Cultivated limbal epithelial cells during the first and second growth generation can keep cornea epitheliam property and high proliferative ability.

关 键 词：角膜缘上皮细胞 培养 生物学特性 

分 类 号：R77[医药卫生—眼科]