肺癌细胞与血管内皮细胞的粘附力学的研究  

An investigation on the mechanical properties of adhesion between lung cancer cellsand endothelial cells

作　　者：沈庆[1] 蒋幼凡[1] 薛亚梅[1] 吴泽志[2] 

机构地区：[1]重庆医科大学第二临床学院呼吸内科,重庆400010 [2]重庆大学生物工程学院,重庆400044

出　　处：《中国医学物理学杂志》2003年第2期112-114,118,共4页Chinese Journal of Medical Physics

摘　　要：目的:本文力图从生物力学角度揭示在卡介菌多糖核酸(BCG-PSN)作用下,肺癌细胞与人脐静脉血管内皮细胞(HUVEC)粘附力学特征,并探讨这一粘附过程与肺癌细胞粘附分子slex的相关关系。方法:采用微管吸吮技术定量测定肺癌细胞与HUVEC的粘附力(Fa);并结合流式细胞仪分析在BCG-PSN作用下,肺癌细胞粘附分子配体slex表达情况。结果:(1)肺癌细胞与HUVEC的粘附力(Fa)在未使用BCG-PSN以前PG细胞大于PAa细胞,用BCG-PSN后,其在PAa细胞呈浓度依赖性及时间依赖性下降,在PG细胞表现为在50μg/ml及以上浓度组粘附力呈明显浓度依赖性下降。各时相点表现为在开始时呈时间依赖性下降,至72小时出现反弹。(2)经BCG-PSN处理后,PAa细胞slex表达呈浓度依赖性下降,PG细胞slex表达无显著差异。结论:实验条件下PG细胞较之PAa细胞有较高的粘附力,且两种细胞与内皮细胞的粘附力均受BCG-PSN的抑制,这可能与PG细胞具有较高的血行转移潜能及BCG-PSN的抗肿瘤作用有关。流式细胞分析研究表明,BCG-PSN对肺癌细胞粘附性的抑制环节很可能是粘附分子表达下降所致的粘附强化启动过程。Purpose: To investigate the effect of Bacillus of Calmette and Guerin-Polysaccharide Nucleic Acid (BCG-PSN) on the mechanical properties of adhesion between lung cancer cells (PG cells or PAa cells)and endothelical cells, and the relevance of cell adhesion to the cell adhesion molecules slex. Methods: Micropitte aspiration technique was used to investigate quantitatively the adhesion force (Fa) between lung cancer cells and human umbilical vein endothelial cells (HUVEC). And the expression of slex of lung cancer cells were evaluated by using cytofluorimetric analysis. Results: The adhesion force(Fa) of PG cells to HUVEC were higher than those of PAa cells under the experimental condition. With treatment with BCG-PSN, the values of the adhesion force of PAa cells to HUVEC decreased monotonously in a concentration-and time-dependentmanner. For PG cells, treatment with in a concentration of 50 μg/ml or higher resulted in a monotonous and continuous decrease of the cell adhesion forces. And the adhesion force to HUVEC decreased continuously with the duration of treament from 30 minutes to 48 hours, but they in creased significantly again in 72 hours past treatment. The expression of slex on PAa cells decreased which was concentration- or dose- dependent upon treatment with BCG-PSN. Whereas no statistically significantly change was found in PG cell. Conclusion: The adhesion force of PG cells to HUVEC were higher than that of PAa cells under the experimental condition. The adhesion force of both of the lung cancer cells to HUVEC can be inhibited by BCG-PSN. These results might be relative to the high metastatic potential of PG cells through blood circulation, and to the antitumor action of BCG-PSN. Our experiments make know that the inhibitory effects of BCG-PSN on the adhesion of lung cancer cells to HUVEC might be due to interference in the initiation of attchment strengthening by down-regulating the expression of cell adhesion molecules on the surface of lung cancer cells.

关 键 词：肺癌细胞 血管内皮细胞 粘附力学 卡介菌多糖核酸 粘附分子 流式细胞仪 生物力学 

分 类 号：R734.2[医药卫生—肿瘤]