降纤酶对大鼠局灶性脑缺血的影响  

Effect of defibrase on focal cerebral ischemia in rats

作　　者：武小玲[1] 王振金[1] 尹岭[2] 冯加纯[3] 饶明利[3] 吕传真[4] 黄如训[5] 

机构地区：[1]中国人民解放军251医院神经内科,河北张家口075000 [2]中国人民解放军301医院神经病学研究所,北京100853 [3]白求恩医科大学第一临床学院神经内科,吉林长春130021 [4]复旦大学医学院神经病学研究所,上海200040 [5]中山医科大学附属第一医院神经内科,广东广州510080

出　　处：《中国新药与临床杂志》2003年第5期273-276,共4页Chinese Journal of New Drugs and Clinical Remedies

基　　金：卫生部科学基金课题 (98 2 390 )

摘　　要：目的 :观察降纤酶 (defibrase)对大鼠局灶性脑缺血性损伤的治疗作用。方法 :雄性Wistar大鼠 ,采用大脑中动脉内线栓法诱导局灶性脑缺血 ,60只局灶性脑缺血大鼠 ,随机分成 1 0组 ,每组 6只 ,其中 5组大鼠分别在缺血后 0 .5 ,3 ,6,9,1 2h静脉注射降纤酶 ,8U·kg-1,另 5组大鼠分别在相同时间点静脉注射等量生理盐水对照 ,另设 1 0鼠作假手术组。各组大鼠均在手术后 2 4h进行神经功能评分 ,然后处死大鼠 ,标本用苏木精曙红染色 (HE)及氯化三苯基四氮唑(TTC)染色 ,观察梗死灶大小、病理改变。结果 :缺血后 0 .5 ,3h静脉给药治疗组神经功能评分比对照组明显改善 ,分别为 (0 .8±s 0 .5 )分 ,(1 .3± 0 .5 )分和 (3 .0± 0 .9)分 ,(3 .2± 1 .0 )分 ,脑梗死灶体积比对照组明显减小 ,分别为 (1 1 9±3 9)mm3 ,(1 41± 62 )mm3 和 (2 91± 5 6)mm3 ,(2 97± 3 9)mm3 ,差别有非常显著意义 (P <0 .0 1 ) ;缺血区病理改变也轻于对照组。缺血后 6,9,1 2h给药组神经功能评分、脑梗死灶体积与对照组比较均无明显差异 (P >0 .0 5 ) ,治疗组与对照组镜下均见点状出血 ,但未发现治疗组增加脑梗死后出血率。结论 :早期静脉应用降纤酶有效缩小脑梗死灶体积 ,且不增加出血率。AIM: To observe the therapeutic effect of defibrase after focal cerebral ischemia in male Wister rats. METHODS:Intraluminal suture method was used to develop reversible middle cerebral artery occlusion (MACO). Sixty focal cerebral ischemia rats were randomly divided into ten groups(one group six rats). Five groups were injected intravenously Hai wang defibrase 8 U·kg 1 at 0.5, 3,6,9,12 h after cerebral ischemia. The other five groups (control groups) were injected intravenously equivalent saline at the same time. Clinical neurological deficits scale were completed at 24 h after MCAO . Then all rats were killed. Cerebral infarction was examined microscopically in HE stained sections. Infarction volume was measured by using 2,3,5 triphenyltetrazolium chloride (TTC) staining. RESULTS:The rats were injected intravenously defibrase in 0.5, 3 h after MCAO. Their clinical neurological deficits scale was 0.8 ± s 0.5, 1.3±0.5, and 3.0±0.9, 3.2±1.0, respectively. The infarction volume was (119±39) mm 3, (141±62)mm 3, and (291±56)mm 3, (297±39)mm 3, respectively. Which were all significantly reduced in comparison with the control groups( P <0.01).Pathologic change was also significantly improved. There were no statistically significant differences at 6, 9,12 h after MCAO. Both treating groups and the control groups were found punctual hemorrhage under the microscop, but cerebral hemorrhage wasn't increased in defibrase treatment group. CONCLUSION: Defibrase can reduce the infarction volumn without the increase of hemorrhage at cerebral ischemia early stage.

关 键 词：局灶性脑缺血 降纤酶 苏木精曙红染色法 氯化三苯基四氮唑染色法 脑梗死灶体积 出血率 

分 类 号：R743.31[医药卫生—神经病学与精神病学]