糖皮质激素对人卵巢癌细胞系(3AO)的诱导分化作用  

Effects of glucocorticoid on differentiation of 3AO: a human ovarian carcinoma cell line

作　　者：徐明娟[1] 崔英[1] 方国恩[1] 宋亮年[2] 王昭梅[1] 

机构地区：[1]第二军医大学长海医院妇产科,上海200433 [2]第二军医大学基础医学部病理生理学教研室

出　　处：《上海医学》2003年第4期265-267,共3页Shanghai Medical Journal

摘　　要：目的　观察不同浓度的地塞米松 (Dex)对人卵巢癌细胞系 ( 3AO)增殖及分化的影响 ,在 3AO细胞中是否有糖皮质激素受体 (GR)的表达。方法　以不同浓度Dex( 1× 10 -10 ～ 1× 10 -6 mol/L)处理培养 3AO细胞后 ,采用活细胞计数法观察细胞增殖情况 ,采用氨基安替比林法测定碱性磷酸酶 (AKP)活性 ;采用酶联免疫法测定 3AO细胞分化标志抗原CA12 5水平的变化 ;测定GR拮抗剂RU486作用于 3AO细胞后能否阻断糖皮质激素的效应 ,用放射配体结合分析法和定量RT PCR法检测 3AO细胞中GR的表达。结果　Dex对 3AO细胞的增殖有明显抑制作用 ,1× 10 -6 mol/LDex作用 5d后活细胞计数为对照组的 5 6.78% ,同时伴有细胞形态的变化 ;Dex还可使 3AO细胞AKP活性增高 ,这种作用具有时间和剂量依赖性 ;1× 10 -6 ～ 1× 10 -10 mol/LDex作用于3AO细胞后 ,明显抑制卵巢癌细胞标志抗原CA12 5的表达 ;RU486可完全阻断Dex对 3AO细胞活性的诱导作用 ;在细胞中存在高亲和力、低容量GR。结论　Dex对 3AO细胞有明显的增殖抑制和诱导分化作用 ,在 3AO细胞中存在GR ,Dex调节Objective To study the effects of dexamethasone (Dex), a synthetic glucocorticoid, on the differentiation of a human ovarian carcinoma cell line (3AO) and the presence or absence of expression of glucocorticoid receptor(GR). Methods 3AO cell proliferation was evaluated by viable cell count and morphological cellular changes, alkaline phosphatase(AKP) activity was determined as described; tumor marker CA125 expression was measured by ELISA. The expression of glucocorticoid receptor in 3AO cells was further studied utilizing radiologic and binding assay.Results Dex inhibited the proliferation of 3AO cells accompanied by morphological changes in a dose-dependent manner. AKP activity increased significantly after treatment with Dex (10 -6 mol/L) for 1～5 d. Dose-response studies revealed that the AKP activity of 3AO cells increased progressively as an effect of Dex concentrations.Furthermore, the expression of tumor marker CA125 in 3AO cells was suppressed in a dose- and time- dependent manner.Conclusion Glucocortocoid plays an important role in the reglulation 3AO cell proliferation and differentiation, and the cellular effects of Dexamethasone was mediated by the presence of GR on the 3AO cells.

关 键 词：糖皮质激素 人卵巢癌 细胞系 诱导分化 地塞米松 酶联免疫法 卵巢肿瘤 肿瘤标志抗原 

分 类 号：R737.31[医药卫生—肿瘤]