急、慢性乙醇处理后大鼠伏核内cAMP反应元件结合蛋白磷酸化的变化  

Changes in the phosphorylation of cAMP response element binding protein in the rat nucleus accumbens after acute and chronic ethanol administration

作　　者：李菁[1] 李跃华[1] 张小虎[2] 朱学江[2] 戈应滨[2] 袁孝如[2] 

机构地区：[1]南京医科大学病理学与病理生理学系,南京210029 [2]南京医科大学生理学系,南京210029

出　　处：《生理学报》2003年第2期147-152,共6页Acta Physiologica Sinica

摘　　要：采用免疫组织化学的方法 ,检测急性、慢性乙醇作用及戒断后大鼠伏核内cAMP反应元件结合蛋白(cAMPresponseelementbindingprotein ,CREB)磷酸化的变化。结果显示 ,急性腹腔注射乙醇后 15min ,伏核内磷酸化CREB(phospho CREB ,p CREB)蛋白明显增加 ,3 0min后达高峰 ,至 1和 6h后仍明显高于对照组。而慢性饮乙醇溶液显著降低大鼠伏核内 p CREB蛋白含量 ,在撤除乙醇后 2 4、72h时 ,伏核内p CREB蛋白含量仍明显较低 ,戒断后 7d ,恢复到正常水平。结果表明 ,急性乙醇处理增加伏核内CREB磷酸化作用 ,而慢性乙醇作用则降低伏核内CREB磷酸化作用 。To define the molecular basis of ethanol dependence, changes in the phosphorylation of cAMP response element binding protein (CREB) in the nucleus accumbens of rats after acute and chronic ethanol administration were detected using immunohistochemistry. The results demonstrate that the expression of phospho-CREB (p-CREB) protein in the rat nucleus accumbens significantly increased after 15 min of acute ethanol exposure, reaching a peak at 30 min after ethanol administration. The increment remained after 1 or 6 h of ethanol exposure compared to the control rats. In contrast, chronic intake of ethanol solution obviously decreased the expression of p-CREB protein compared to the control rats. The decrement remained 24 h or 72 h after ethanol withdrawal, and returned to the control levels after 7 d of ethanol withdrawal. The results suggest that an acute ethanol administration led to an increase in the phosphorylation of CREB in the nucleus accumbens, but chronic ethanol administration produced a decrement, which is possibly one of the molecular mechanisms of alcohol dependence.

关 键 词：乙醇 伏核 CAMP反应元件结合蛋白 

分 类 号：R363[医药卫生—病理学]