维甲酸、三氧化二砷诱导NB4细胞TRAIL基因表达的研究  

Study on the induction of TRAIL gene expression in NB4 cells by retinoid acid and arsenic trioxide

作　　者：王玲玲[1] 张茂宏[2] 

机构地区：[1]山东大学护理学院,济南250012 [2]山东大学齐鲁医院血液科

出　　处：《临床血液学杂志》2003年第3期130-131,共2页Journal of Clinical Hematology

摘　　要：目的:研究全反式维甲酸(ATRA)或三氧化二砷(As_2O_3)诱导急性早幼粒细胞白血病(APL)NB4细胞肿瘤坏死因子相关的凋亡诱导配体(TRAIL)基因的表达及其治疗APL的机制。方法:采用RT-PCR检测TRAIL基因表达的变化。结果:10^(-6)mol/L的ATRA作用6h或10^(-6)mol/L的As_2O_3作用12h、即可诱导TRAIL基因表达。结论:ATRA或As_2O_3能诱导NB4细胞TRAIL基因表达,TRAIL基因可能以类似“旁分泌”的作用方式杀伤NB4细胞。诱导TRAIL基因介导的细胞凋亡可能是ATRA或As_2O_3治疗APL的机制之一。Objective:To explore the induction of TRAIL gene expression by ATRA or As2O3 in NB4 cells and its theraputic value. Method: RT-PCR was used to determine TRAIL gene expression. Result: The expression of TRAIL gene was induced after exposure to 10- 6mol/L ATRA for 6 h or 10-6mol/L As2O3 for 12 h. Conclusion: ATRA or AszO3 could upregulate TRAIL gene expression in NB4 cells, TRAIL might kill NB4 cells in a paracrine mode of action. Thus induction of TRAIL-mediated death signaling may contribute to the theraputic value of ATRA or As2O3.

关 键 词：基因 肿瘤坏死因子相关的凋亡诱导配体 细胞凋亡 维甲酸 三氧化二砷 NB4细胞系 

分 类 号：R730.3[医药卫生—肿瘤]