微生物发酵秸秆产氢的气相色谱分析  

Determination of hydrogen produced by microbe-fermented stover by gas chromatography

作　　者：赵静玫[1] 刘波[1] 孙艳[1] 徐■俐 王海岩[1] 李雪驼[1] 

机构地区：[1]中国科学院大连化学物理研究所生物技术部,大连116023

出　　处：《分析仪器》2003年第2期29-31,共3页Analytical Instrumentation

基　　金：科研创新基金项目 (k2 0 0 0A9)

摘　　要：利用气相色谱法跟踪测定发酵液产氢含量 ,对微生物发酵农作物秸秆产氢菌种进行筛选。实验结果表明 ,C3(产气肠杆菌 ) ,M1(麦芽糖假丝酵母菌 ) ,H4[C3 +M 1+D2 (丁酸梭菌 )混合菌 ]是较好的产氢菌种 ,所产氢气的体积分数分别为C3( 6 0 0 4% ) ,H4( 5 9 4 1% ) ,M 1( 11 2 7% )。色谱柱为TDX 0 1( 2m× 0 .5mmid) ,鉴定器为TCD ,柱温 10 0℃ ,载气Ar 40mL/min。方法操作简单 ,快速方便 ,重现性好 ,灵敏度高。The hydrogen produced by liquor of fermented stover was determined by gas chromatography for the selection of microbe species to be used to ferment stover to produce hydrogen. The experimental results demonstrate that the C3 (enterobacter aerogenes), M1 (candida alosa), and H4, a mixture of C3, M1 and D2 (clostridium butylicum), are the better hydrogen producing strains. The volume fractions of hydrogen produced by C3, H4 and M1 are 60.04%, 59.41% and 11.27%, respectively. The experimental conditions are TDX-01 column (2m×0.5mm id), temperature 100℃, TCD, carrier gas (He) 40 mL/min. The method is easy, rapid, accurate and sensitive.

关 键 词：微生物发酵 秸秆 产氢 气相色谱 菌种 

分 类 号：O657.7[理学—分析化学]