左旋咪唑激发大鼠实验性变态反应性脑脊髓炎的初步观察  

The primary research on experimental allergic encephalomyelitis induced by levamisole in rats

作　　者：李勇[1] 李剑敏[2] 郑荣远[1] 杨学志[1] 邵蓓[1] 何金彩[1] 金嵘[1] 潘建春[3] 

机构地区：[1]温州医学院第一附属医院神经内科,浙江温州325027 [2]温州医学院第一附属医院病理科,浙江温州325027 [3]温州医学院药理学院,浙江温州325000

出　　处：《温州医学院学报》2003年第2期82-84,共3页Journal of Wenzhou Medical College

基　　金：浙江省重点学科基金资助项目 (xxf990 0 5 )

摘　　要：目的 :观察左旋咪唑 (LMS)激发大鼠实验性变态反应性脑脊髓炎 (EAE)的作用。方法 :将Wistar大鼠分为 5组 :Control组、GPSCH(豚鼠全脊髓匀浆 ) +CFA(完全福氏佐剂 )组、LMS组、LMS +CFA组和CFA组 ,采用动物行为学、常规HE和LFB染色法观察动物的发病情况与中枢神经系统 (CNS)的病理变化情况。结果 :①LMS +CFA组 2 /10和GPSCH +CFA组 5 /10大鼠出现典型的EAE行为学改变、CNS炎性细胞浸润和髓鞘脱失。②CFA组 8/10大鼠出现佐剂性关节炎症状 ,未见CNS炎性细胞浸润和髓鞘脱失。③LMS组和Control组都未见动物行为学和CNS病理学改变。结论 :LMS +CFA可直接激发EAE 。Objective:To observe the effect of experimental allergic encephalomyelitis induced by levamisole in rats.Methods:Fifty Wistar rats were randomly divided into five groups:control group,guinea pig spinal cord homogenate(GPSCH) plus complete freund's adjuvant(CFA) group,levamisole group,levamisole plus CFA group and CFA group. The diet,movement,body weight of Wistar rats were daily examined,CNS tissues were stained with hematoxylin eosin and luxol fast blue.Results:①Two of ten rats in levamisole plus CFA group and five of ten in GPSCH plus CFA group demonstrated neurological signs of typical EAE, with parenchymal infiltration and perivascular cuffing with mononuclear cells, and demyelination in the white matter.②Eight of ten in CFA group demonstrated the signs of the adjuvant arthritis,without demyelination and infiltration of mononuclear in CNS.③No infiltration of mononuclear and myelin damages in CNS were noted in all rats of LMS and control groups.Conclusion:Levamisole plus CFA can directly induce EAE in wistar rat.

关 键 词：左旋咪唑 实验性变态反应性脑脊髓炎 动物模型 实验研究 

分 类 号：R744.3[医药卫生—神经病学与精神病学]