哺乳仔猪中伪狂犬病病毒鄂A株对细胞凋亡的诱导或抑制  

Induction or Inhibition of Apoptosis by Pseudorabies Virus Ea Strain in Suckling Piglets

作　　者：李祥敏[1] 钱平[1] 石德时[1] 金梅林[1] 蓝盛银[2] 陈焕春[1] 

机构地区：[1]华中农业大学动物医学院动物病毒室,武汉430070 [2]华中农业大学生命科学技术学院分子细胞生物学研究室,武汉430070

出　　处：《生物化学与生物物理学报》2003年第4期325-330,共6页

基　　金：国家高技术研究发展计划 (863计划 )资助项目 (No .2 0 0 1AA2 13 0 5 1) ;湖北省自然科学基金资助项目 (No .2D0 1ABD110 )~~

摘　　要：通过人工感染伪狂犬病病毒阴性的健康仔猪 ,取实验组和阴性对照组仔猪的咽部扁桃体、胸腺、颈部淋巴结、腹股沟淋巴结、大脑、小脑、嗅球、三叉神经节等组织作样本。通过电子显微镜观察、DNA梯谱和原位末端脱氧核苷酸标记等试验进行细胞凋亡分析 ,结果发现实验组仔猪的淋巴组织细胞呈现典型的细胞凋亡的特征 ,而神经组织中无。这一结果表明 :伪狂犬病病毒感染诱导大量淋巴细胞凋亡 ,造成仔猪免疫功能低下 ,导致大量死亡 ,这可能是伪狂犬病病毒急性感染致病机制之一 ;同时也证实伪狂犬病病毒通过抑制神经细胞的凋亡 ,在神经组织中建立潜伏感染并终生带毒。To study induction or inhibition of apoptosis by pseudorabies virus (PrV) Ea strain in suckling piglets, Aujeszky’s disease was replicated by artificially inoculating 15 day-old piglets with PrV-Ea strain. Various tissue sections, such as lymphoid tissues and nervous tissues were collected. Transmission electron microscopy, DNA fragmentation assay and in situ terminal-deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) staining were carried to analyze apoptotic cells. It was shown that piglets infected with PrV-Ea strain took on typical clinical symptoms and apoptotic cells were found in lymphoid tissues but not in nerve tissues. The results indicated that the PrV infection caused apoptosis in a big number of lymphatic cells, thus leading to death of suckling pigs due to lowered immune function. It might play an important role in PrV’s attack on immune system. A latent infection of pseudorabies virus was established in the neuronal tissue cells by inhibition of nervous cell apoptosis; this might be the way for PrV to establish latent infection, leading to sporadic recurrence of the disease.

关 键 词：哺乳仔猪 伪狂犬病病毒 鄂A株 细胞凋亡 诱导 抑制 淋巴组织细胞 神经组织细胞 致病机制 

分 类 号：S852.65[农业科学—基础兽医学]