内蒙古锡盟地区蒙古族人群VWA、FGA、PENTAE基因座的群体遗传学研究  

THE DETECTION OF POPULATION GENETICS OF VWA,FGA,PENTAE LOCI IN MONGOLIAN OF INNER MONGOLIA

作　　者：李东霞[1] 乌云高娃 李彩萍[3] 王凤歧[3] 邱琳[3] 毕力夫[1] 苏秀兰[1] 

机构地区：[1]内蒙古医学院分子生物学研究中心,内蒙古呼和浩特010059 [2]内蒙古锡盟医院 [3]内蒙古医学院第一附属医院内科

出　　处：《内蒙古医学院学报》2003年第1期4-8,共5页Acta Academiae Medicinae Neimongol

基　　金：内蒙古自然科学基金项目 ( 2 0 0 1 0 90 6-1 0 )

摘　　要：目的 :了解内蒙古锡盟地区蒙古族人群 VWA、FGA、PENTA E三个 STR位点的遗传多态性分布 ,获得相应多态位点的群体遗传学数据。方法 :采用多重 PCR扩增技术和聚丙烯酰胺凝胶电泳方法对 2 63名蒙古族个体进行了研究。结果 :VWA、FGA、PENTA E三个位点分别检出 9、1 0、2 2个等位基因 ,多态性分布均符合Hardy-Weinberg平衡定律。杂合度 ( H)分别为 0 .81 1 5、0 .8487、0 .91 46,多态信息量 ( PIC)分别为 0 .782 5、0 .82 98、0 .90 64。结论 :上述三个位点均具有较高的杂合度和多态信息量 ,是较理想的遗传标记系统。同时揭示了蒙古族人群三个 STR位点的遗传多态性分布 。Objective: To study the distribution of genetic polymorphism of VWA, FGA, PENTA E loci in Mongolia population and construct genetic database. Methods: Multiplex PCR and polyacrylamide gel(PAG) electrophoresis were used to investigate the polymorphism of 3 STR loci in 263 cases of Mongolia population. Results: 9, 10, 22 alleles were observed at these 3 STR loci. The genotypes distribution in Mongolia population was in accordance with Hardy\|Weinberg equilibrium. The expected heterozygosities(H) for 3 loci were 0.8115, 0.8487, 0.9146 respectively, the polymorphism information contents(PIC) were 0.7825, 0.8298, 0.9064 respectively. Conclusion: The results demonstrate that these 3 STR loci can be used as genetic markers and provide valuable data which are beneficial to study the population genetics of the three STR loci in Mongolia population of Inner mongolia.\;

关 键 词：聚合酶链反应 短串联重复序列 遗传多态性 内蒙古蒙古族群体 VWA FGA PENTAE 

分 类 号：R394.5[医药卫生—医学遗传学]