肝癌组织中survivin蛋白表达的意义  

Expression of survivin protein in hepatocellular carcinoma tissues and its relationship with clinical pathological features and prognosis

作　　者：陈涛[1] 贾玉容[1] 田伏洲[1] 蔡忠红[1] 李广阔[1] 

机构地区：[1]成都军区总医院全军普通外科中心,四川省成都市610083

出　　处：《世界华人消化杂志》2003年第4期411-414,共4页World Chinese Journal of Digestology

摘　　要：目的:研究survivin蛋白在人肝癌组织中的表达与临床病理特征及预后的关系。方法:应用免疫组织化学染色对48例肝癌中survivin蛋白与增生细胞核抗原(PCNA)的表达情况进行检测,采用TUNEL方法检测细胞凋亡,结合临床病理特征及预后进行分析。结果:48例肝癌中survivin蛋白表达阳性率为64.6％。Edmondson分级Ⅰ-Ⅱ级患者survivin蛋白表达明显低于Ⅲ一Ⅳ级患者(39.1％vs88.0％,P=0.013)。survivin蛋白表达阳性者肝癌细胞增生凋亡比显著高于表达阴性者(1.8vs1.1,P=0.045)。survivin蛋白表达阴性患者与表达阳性患者比较,1a生存率差异无显著意义,但3a生存率前者显著高于后者(70.6％vs35.5％,P=0.011)。结论:survivin蛋白对破坏肝癌细胞增生凋亡平衡、促进肝癌细胞快速增生具有重要作用,survivin蛋白的表达可以作为预后不良的重要指标。AIM:TO investigate the expression of survivin protein in human hepatocellular carcinoma (HCC) and its relationship with clinical features and prognosis of patients with HCC. METHODS:The expression of survivin protein and the proliferation of tumor cells marked by PCNA in 48 cases of HCC were assessed by immunohistochemical method. TUNEL method was used to detect apoptosis. RESULTS:The survivin protein was expressed in 31 of 48 cases of HCC (64.6 %). Expression of survivin protein was significantly higher in those of Edmondson grade Ⅲ-Ⅳ than in those of grade Ⅰ-Ⅱ (88.0 % vs 39.1%, P=0.013). The ratio of proliferative index to apoptotic index was significantly higher in HCC with positive survivin expression than that with negative survivin expression (1.8 vs 1.1, P =0.045). The survival rate of three years of patients with positive survivin expression was significantly lower than that of patients with negative suwivin expression (70.6 % vs 35.5 %, P=0.011). CONCLUSION:The expression of survivin may play an important role in breaking the balance of proliferation and apoptosis of HCC cells and is closely associated with prognosis of patients with HCC.

关 键 词：肝癌组织 SURVIVIN蛋白 临床病理特征 预后 免疫组织化学 肝癌 增生细胞核抗原 细胞凋亡 

分 类 号：R735.7[医药卫生—肿瘤] R730.3[医药卫生—临床医学]