成牙本质细胞中上游刺激因子1表达的原位杂交研究  

In situ Hybridization Analysis of USF1mRNA Expression in Odontoblasts

作　　者：吴礼安[1] 文玲英[1] 杨富生[1] 刘勇[1] 

机构地区：[1]第四军医大学口腔医学院儿童口腔科,陕西西安710032

出　　处：《上海口腔医学》2003年第2期120-122,共3页Shanghai Journal of Stomatology

摘　　要：目的检测、证实成牙本质细胞中上游刺激因子1(USF1)mRNA的表达。方法用前期制备的T—USF1质粒作模板,借助特异性引物PCR扩增USF1cDNA片段,回收、随机引物法地高辛标记;培养成牙本质细胞MDPC-23,制备细胞爬片,原位杂交方法检测。结果成牙本质细胞胞浆中有较强的蓝紫色颗粒。结论首次证实体外培养的成牙本质细胞中有USF1mRNA表达,为深入研究USF1分子对成牙本质细胞生长、分化和矿化影响提供了实验依据。Objective To detect and verify USF1mRNA expression in odontoblasts.Methods T-USF1clone prepared previously was used as template and the desired USF1cDNA segment was amplified by PCR with specific primers.The segment was labeled with digoxin as a probe and in situ hybridization was performed on the mounting of odontoblasts.Results Evidently positive staining was shown in the cytoplasm of odontoblasts.Conclusion We verified the expression of USF1mRNA in odontoblasts for the first time and provided evidence for further research.

关 键 词：成牙本质细胞 上游刺激因子1 原位杂交 MRNA PCR 细胞生长 体外培养 

分 类 号：R780.2[医药卫生—口腔医学]