棘尾虫酸性磷酸酶的定位及诱导表达  

Localization of ACPase and its expression by induction in Stylonychia mytilus

作　　者：陈瑛[1] 邱子健[1] 隋淑光[1] 史新柏[1] 

机构地区：[1]哈尔滨师范大学生物系,哈尔滨150080

出　　处：《动物学报》2003年第2期218-223,共6页ACTA ZOOLOGICA SINICA

基　　金：国家自然科学基金资助项目 (No .3 0 0 70 0 91);黑龙江省自然科学基金资助项目~~

摘　　要：本文利用电镜对贻贝棘尾虫 (Stylonychiamytilus)体内酸性磷酸酶的定位进行了观察。发现其体内除含有溶酶体性的酸性磷酸酶 (ACPase)外 ,在纤毛内部还存在非溶酶体性的ACPase。另外 ,本文还利用光镜和酶细胞化学技术观察并比较了棘尾虫年轻态和衰老态个体体内ACPase在食物诱导下的表达量随时间发生的动态变化和差异 。Acid phosphatase (ACPase) is a phosphomonoesterase, that is widely dis tributed in nature, mainly localized on lysosomes, Golgi complexes, and the endo plasmic reticulum. We did two experiments on acid phosphatase (ACPase) in young and ageing cells of Stylonychia mytilus. Ageing cells came from an emicronuc leat ed clone which was obtained in Sep. 1988 and had undergone more than 2000 asexua l cell divisions up to the present experiment. Young cells came from a new clone which originated recently from an exconjugant and had passed through only about 100 cell divisions. The two experiments were:Ⅰ. Observe the localization of ACPase in the young cells with enzyme cytochemical techniques and electron micro scopy. After being fed for 30 min, young cells were fixed with glutaraldehyde ( 2 5%) and paraformaldehyde (4%) in sodium cacodylate buffer and incubated in in cubating liquid (6 ml distilled water +2 ml tris maleicacid +2 ml lead nitr ate +2 5 mg β sodium phosphoglycerol) at 37 ℃ for 2 5-3 hr, re fixed for 10 mi n in 2 per cen t osmium tetroxide, gradient dehydrated in acetone, embeded in EPON812 and secti oned with a Leica ultramicrotome. Sections were viewed with a JEOL 1200 transmi s sion electron microscope. In the control experiment redistilled water replaced phosphoglycerol in the incubating liquid. Electron microscopy revealed that ther e are three positive ACPase reaction sites in Stylonychia mytilus : (1) in th e cilia; (2) on the lithosomes (Hausmann et al .,1996) and tubular bodie s i ns ide the digestive channels (Kaul et al .,1982); (3) in the food organisms. The ACPas e on the tubular bodies and in the food organisims inside digestive channels is lysosomic ACPase. This has been described by Kaul et al . (1982), who suggest ed t hat this was involved in the digestive process of Stylonychia . Lithosomes ar e sp herical deposits produced from concentric layers of organic and inorganic materi al inside the digestive channels. Hausmann considered it to be organic crysta

关 键 词：棘尾虫 酸性磷酸酶 衰老 酶活性 酶含量 酶细胞化学 定位 诱导表达 

分 类 号：Q55[生物学—生物化学]