超声波辅助处理对发根农杆菌介导的苦豆子遗传转化的影响  

The effect of additional ultrasonic treatment on genetic transformation of Sophora alopecuroides mediated by Agrobacterium rhizogenes

作　　者：赵东利[1] 陈红波[1] 聂秀菀[1] 王新宇[1] 郑国锠[1] 

机构地区：[1]兰州大学细胞生物学研究所

出　　处：《西北植物学报》2003年第3期468-472,共5页Acta Botanica Boreali-Occidentalia Sinica

摘　　要：以发根农杆菌转化苦豆子的子叶和下胚轴外植体,结果表明,辅助以超声波处理有助于转化率的提高,当超声波(功率120W,震荡频率50kHz)处理25min时,转化率达到最高峰(子叶为83.7％,下胚轴为39.1％),分别高于对照(14.4％和9.8％)69.3％和29.3％。在此条件下,进一步添加AS100μmol/L,可使转化率再进一步提高9.9％和7.6％。The effect of additional ultrasonic treatment on transformating rate of Sophora alopecuroides me-diated by Agrobacterium rhizogenes was studied in the present paper. The highest rates,83. 7% for cytole-don and 39. 1% for hypocotyl explants,were achieved after 25 min of treatment (120 W,50 kHz),being 69. 3% and 29. 3% higher than control (14. 4% and 9. 8%). Meanwhile,the supplement of 100 μmol/L acetosyringone (AS) to A. rhizogenes suspension could further increase the rate by 9. 9% and 7. 6% for cy-toledon and hypocotyl explants,respectively.

关 键 词：苦豆子 超声波处理 发根农杆菌 遗传转化 乙酰丁香酮 药用植物 

分 类 号：S567.19[农业科学—中草药栽培]