抗视网膜母细胞瘤细胞株SO-Rb_(50)单克隆抗体相应抗原的提取  

Separation of SO-Rb_ (50)cells antigen corresponding to the monoclonal antibody of anti-retinoblastoma

作　　者：应方微[1] 李永平[1] 唐松[1] 钟秀风[1] 林健贤[1] 郑健樑[1] 潘苏华[1] 易玉珍[1] 

机构地区：[1]中山大学中山眼科中心病理室,广州510060

出　　处：《中华眼底病杂志》2003年第3期152-155,共4页Chinese Journal of Ocular Fundus Diseases

基　　金："2 1 1"工程资助项目 (980 0 7);国家自然科学基金资助项目(39870 80 1 );广东省自然科学基金资助项目 (980 1 1)

摘　　要：目的　从 SO- Rb50 细胞中提取出抗视网膜母细胞瘤单克隆抗体相应的抗原。　方法　用离子交换层析法从 SO- Rb50 细胞中初步分离抗原 ,并利用抗视网膜母细胞瘤单克隆抗体以 dot blotting鉴定抗原 ,然后以十二烷基硫酸钠 -聚丙烯酰胺凝胶电泳分离纯化出抗原目的蛋白条带 ,用 Westein blotting检测其相对分子质量。　结果　从 SO- Rb50 细胞裂解液的总蛋白中分离出一条抗原目的蛋白条带 ,其相对分子质量约为 83× 10 3。　结论　从 SO- Rb50 细胞裂解液中能够提取出抗视网膜母细胞瘤单克隆抗体对应的抗原。Objective To separate the SO-Rb 50cells antigen corresponding to the monoclonal antibody of anti-retinoblastoma. Methods The antigen corresponding to the monoclonal antibody of anti-retinoblastoma was separated elementarily by ion-exchange chromatography, and was identified by dot-blotting using the monoclonal antibody of anti-retinoblastoma. The target protein band of the antigen was separated in light of sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Results A special unmixed band of SO-Rb 50cells antigen was separated with the relative molecular weight of 83×103. Conclusion The antigen corresponding to the monoclonal antibody of anti-retinoblastoma could be separated from SO-Rb 50cells.

关 键 词：抗视网膜母细胞瘤细胞株 SO-RB50 单克隆抗体 抗原 提取 分离 纯化 

分 类 号：R739.7[医药卫生—肿瘤]