血管内皮生长因子反义寡聚脱氧核苷酸对增生性视网膜病变幼鼠血管内皮生长因子表达的影响  

Effect of vascular endothelial cell growth factor (VEGF) antisense oligodeoxynucleotides on expression of VEGF in rats with oxygen-induced retinopathy

作　　者：邓德勇[1] 韩丽荣[1] 吴丹巍[2] 夏风华[1] 彭亚军[1] 孔屹[1] 王惕 

机构地区：[1]第二军医大学长海医院眼科 [2]上海中医药大学附属岳阳医院眼科 [3]上海解放军85医院眼科

出　　处：《中华眼底病杂志》2003年第3期172-174,共3页Chinese Journal of Ocular Fundus Diseases

摘　　要：目的　观察血管内皮生长因子 ( vascular endothelial cell growth factor,VEGF)反义寡聚脱氧核苷酸 ( antisense oligodeoxynucleotides,ASODNs)对增生性视网膜病变幼鼠模型 VEGF表达的影响。　方法　将 30只 Sprague- Dawley( SD)新生幼鼠随机分为正常对照组、用药组及未用药组 ,每组 10只。用高浓度氧诱导用药组及未用药组 SD幼鼠 ,建立增生性视网膜病变幼鼠模型。对用药组幼鼠行 VEGFASODNs球后注射 ,3 d后取各组幼鼠除角膜、巩膜及晶状体外的全部眼内组织及血清 ,通过竞争性酶免疫测定法检测其 VEGF的含量。　结果　用药组幼鼠眼内组织 VEGF含量明显低于未用药组 ( P<0 .0 5 ) ,而与对照组相比差异无显著性的意义 ( P>0 .0 5 ) ;用药组幼鼠血清 VEGF含量与未用药组相比差异无显著性的意义 ( P>0 .0 5 ) ,两者均明显低于对照组 ( P<0 .0 5 )。　结论　 VEGF ASODNs能明显抑制由高浓度氧诱导的增生性视网膜病变幼鼠 VEGF的表达。Objective To evaluate the effect of vascular endothelial cell growth factor (VEGF) antisense oligodeoxynucleotides (ASODNs) on the expression of VEGF in rats with oxygen-induced retinopathy. Methods Thirty newborn Sprague-Dawley (SD) rats were randomly divided into 3 groups:normal control group, disposal group and non-disposed group, The animal models with oxygen-induced proliferative retinopathy were established by raising the rats in hyperoxic environment. Retrobulbar injection was performed with VEGF ASODNs or normal saline on the rats in 3 groups respectively. The intraocular tissues (all the tissues except the cornea, sclera, and lens) and serum were collected, and the expressions of VEGF were determined by using competitive enzyme immunoassay. Results The expressions of VEGF in intraocular tissues of rats in disposal group were significantly lower than those in non-disposed group (P<0.05), and there was no statistical difference between the disposal and normal control group (P>0.05). There was no significant difference of the expressions of VEGF in serum of rats between the disposal and non-disposed group (P>0.05), which were both lower than those in the normal control group (P<0.05). Conclusion VEGF ASODNs could significantly inhibit the expression of VEGF in intraocular tissues.

关 键 词：血管内皮生长因子 反义寡聚脱氧核苷酸 增生性视网膜病变 幼鼠 血管内皮生长因子 表达 影响 

分 类 号：R774.1[医药卫生—眼科]