玻璃体胶原纤维的空间结构及纤溶酶对其空间结构的影响  

Ultrastructure collagen fibril organization in vitreous and the effect of plasmin on it

作　　者：于文贞[1] 黎晓新[1] 鲁崎晤[2] 徐伟[2] 

机构地区：[1]北京大学人民医院眼科,100044 [2]中国科学院生物物理所

出　　处：《中华眼底病杂志》2003年第3期179-181,共3页Chinese Journal of Ocular Fundus Diseases

摘　　要：目的　观察玻璃体胶原纤维的空间结构及纤溶酶对其空间结构的影响。　方法　取新鲜猪眼玻璃体 2 0个 ,随机分 2组 ,一组的 10个玻璃体中分别加入用磷酸盐缓冲液 ( phosphate- buffered saline,PBS)配制的浓度为 3U / ml的人纤溶酶消化液 1ml,另一组的 10个玻璃体中分别加入等量的 PBS作为对照。均置于密闭的容器 ,在 37℃的细胞培养箱中孵育 2 4h,固定剂固定。分别取玻璃体基底部、皮质和中央区的标本进行冷冻断裂、深度蚀刻联合透射电子显微镜检查。　结果　玻璃体的胶原纤维形成有机的网状结构 ,具有稳定的空间构像。各部位纤维密度不均 ,基底部最密集 ,皮质部次之 ,中央区胶原纤维稀疏。纤维之间具有更加细小的纤维丝相连 ,以纤维交汇处为著。经过纤溶酶消化后 ,玻璃体胶原纤维的网状结构破坏。　结论　玻璃体内胶原纤维呈三维空间结构 ,纤溶酶能够破坏玻璃体胶原纤维的网状结构。Objective To reveal the fibrillar network in vitreous and the effect of plasmin on this network. Methods 20 vitreous gels of freshly slaughtered pigs were divided into 2 groups, the gels in first group were digested by 3 U plasmin (3 U/ml) at 37℃ for 24 hours respectively, the second group received the same PBS as control. After digestion, gels were fixed in neutral buffered formalin solution. Samples from vitreous base, cortex and the central region were observed by the technique of freeze etching electron microscopy. Results In vitreous collagen fibril network was in a three-dimensional array, collagen fibril density showed marked differences, central vitreous had the sparse fibril density, the cortex denser and the basal vitreous densest. After digestion by plasmin, the collagen fibrillar network was destructed. Conclusion Collagen fibrils in vitreous present spatial arrangement regularly, plasmin can lead to destruction of the fibrillar network.

关 键 词：玻璃体胶原纤维 空间结构 纤溶酶 影响 冷冻蚀刻 

分 类 号：R776.4[医药卫生—眼科]