尿中核苷检测在胃癌诊断中的意义  

Significance of Urinary Nucleosides in Diagnosis of Gastric Carcinoma

作　　者：陈英杰[1] 郑育芳[2] 王凝芳[3] 吕申[4] 逄涛[2] 杨青[2] 许国旺[2] 

机构地区：[1]大连大学医学院病理学教研室,辽宁大连116622 [2]中国科学院大连化学物理研究所国家色谱中心,辽宁大连116011 [3]大连大学医学院外科教研室,辽宁大连116622 [4]大连医科大学附属第二医院实验中心,辽宁大连116023

出　　处：《癌症》2003年第5期537-540,共4页Chinese Journal of Cancer

基　　金：国家自然科学基金项目(No.29775024);中国科学院知识创新工程领域前沿项目基金项目(No.DICPkaaaabc)

摘　　要：背景与目的:有研究表明,尿中修饰核苷的含量在多数恶性肿瘤患者中有明显升高。本研究拟探讨检测尿中核苷在胃癌诊断中的意义。方法:应用高效液相色谱法分别检测50名正常人与48例胃癌患者尿中15种核苷的水平,48例胃癌患者中有25例同时接受了血清CEA检测。结果:50名正常人和48例胃癌患者尿中15种核苷的平均值分别依次为:Pseu22.91±4.90,34.87±21.41;U0.34±0.32,0.62±0.82;A0.58±0.16,0.96±0.75;C0.17±0.15,0.24±0.19;m5U0.03±0.07,0.07±0.06;I0.26±0.10,0.43±0.36;m1I1.34±0.34,2.44±1.39;ac4C0.75±0.24,1.08±0.72;G0.09±0.04,0.14±0.10;X1.20±0.42,1.90±1.09;m2G0.61±0.16,1.00±0.69;m6A0.04±1.13,0.07±0.08;m1A2.26±0.56,3.71±2.21;m22G1.34±0.27,2.25±1.39;m1G0.80±0.25,1.41±0.86。胃癌患者尿中除m5U外,其余14种核苷的平均值明显高于正常人(P<0.05);次黄嘌呤与肿瘤大小,淋巴结转移正相关(P<0.05);黄嘌呤核苷与肿瘤淋巴结转移正相关(P<0.05)。以其中15种核苷浓度作为数据矢量,结合主成分分析-投影判别法区分正常人和胃癌患者,63%的胃癌患者被识别,识别率大大高于CEA检测(12%)。结论:胃癌患者尿中修饰核苷水平升高,检测尿中修饰核苷对胃癌的初筛有一定的参考意义。BACKGROUND &OBJECTIVE: It was reported that urinary modified nucleosides with abnormally high amounts were found in many cancer patients. This study was designed to investigate the usefulness of urinary nucleosides in the diagnosis of gastric carcinoma. METHODS: The concentrations of 15 kinds of urinary nucleosides from 50 healthy persons and 48 patients with gastric carcinoma were determined by high performance liquid chromatography (HPLC). Of 48 patients with gastric carcinoma, 25 underwent serum CEA examination. RESULTS: The average levels of 14 kinds of urinary nucleosides, m5U excepted, from patients with gastric carcinoma were higher than those from health persons (P< 0.05). Pseu 22.91±4.90,34.87±21.41; U 0.34±0.32,0.62±0.82; A 0.58±0.16,0.96±0.75; C 0.17±0.15,0.24±0.19; m5U 0.03±0.07,0.07±0.06; I 0.26±0.10,0.43±0.36; m1I 1.34±0.34,2.44±1.39; ac4C 0.75±0.24,1.08±0.72; G 0.09±0.04,0.14±0.10; X 1.20±0.42,1.90±1.09; m2G 0.61±0.16,1.00±0.69; m6A 0.04±1.13,0.07±0.08; m1A 2.26±0.56,3.71±2.21; m22G 1.34±0.27,2.25±1.39; m1G 0.80±0.25,1.41±0.86. The level of nucleoside I was positively correlated with the tumor size(P< 0.05). The level of nucleoside X was positively correlated with lymph node metastasis(P< 0.05). Using the concentrations of 15 nucleosides as the data vectors, principal component analysis was applied to classify gastric cancer patients and normal adults, 63%(30/48)of cancer patients were correctly classified, in which the identification rate was higher than that of CEA method (12%). CONCLUSION: Urinary modified nucleoside increased in the patients with gastric carcinoma, and it may be helpful in the diagnosis of gastric carcinoma.

关 键 词：尿 核苷 检测 胃癌 诊断 

分 类 号：R735.2[医药卫生—肿瘤]