APAGE技术在小麦细胞质雄性不育系选育中的应用研究  

Studies on Application of APAGE in Male Sterile Wheat with Alloplamic Cytoplasm

作　　者：王小利[1] 张改生[1] 陈新宏[1] 刘宏伟[1] 王军卫[1] 

机构地区：[1]西北农林科技大学农学院陕西省作物杂种优势研究与利用重点实验室,杨凌712100

出　　处：《Acta Genetica Sinica》2003年第5期465-468,共4页

基　　金：国家自然科学基金 ( 30 170 5 6 7);杨凌农业生物技术育种中心基金资助

摘　　要：利用大量小麦亲本材料和优良品种 (系 )与具有粘果、易变、偏凸和二角山羊草细胞质的小麦雄性不育系杂交 ,筛选出一系列保持系。利用APAGE(酸性聚丙烯酰胺凝胶电泳 )技术对其进行了醇溶蛋白电泳图谱分析 ,发现大部分保持系表现出 1BL 1RS易位系的 1RS醇溶蛋白标记位点Gld1B3。利用细胞学镜鉴 ,发现含有Gld1B3标记位点的保持系均只含有两个随体 ,而不含有Gld1B3标记位点的保持系均含有 4个随体 ,证明了Gld1B3标记位点与两个随体数的一致性。粘、易、偏型不育系育性基本表现一致 ,而二角型不育系除了与前 3种不育系具有相同的保持系以外 ,对某些小麦品种 (系 )还表现出育性特异性。同时还讨论了APAGE技术在快速筛选小麦细胞质雄性不育保持系中的作用 ,为非 1BL 1RS不育系的选育提供了必要的手段。Four male sterile wheat with Aeglops kotschyi,Ae.variabilis,Ae.ventricosa and Ae.bicornis cytoplasm,were crossed with some wheat materials,and from the crosses a series of maintenance lines were selected.The APAGE(acid polyacrylamide gel electrophoresis) analysis indicated that most maintenance lines possessed the gliadin marker Gld1B3 of 1BL/1RS translocation chromosome.The root tip chromosomes of this maintenance lines had two satellite chromosomes,but other maintenance lines without Gld1B3 marker had the same four satellite chromosomes as other common wheat.Three male sterile wheats with Aeglops kotschyi,Ae.variabilis,Ae.ventricosa cytoplasm had the same fertility,but the male sterile wheat with Ae.bicornis cytoplasm could produce male sterile.Meantime,the paper discussed application of APAGE technology in breeding of non-1BL/1RS male sterile wheat.

关 键 词：异质小麦 1BL/lRs易位染色体 醇溶蛋白 随体 

分 类 号：S512.1[农业科学—作物学]