形目12种鸟类线粒体细胞色素b基因序列差异及其系统发育关系  

Sequence Variation of Mitochondrial Cytochrome b Gene and Phylogenetic Relationships among Twelve Species of Charadriiformes

作　　者：陈晓芳[1] 王翔[1] 袁晓东 汤敏谦 李玉祥 郭玉梅 李庆伟[1] 

机构地区：[1]辽宁师范大学生命科学学院,大连116029 [2]宝生物工程大连有限公司,大连116600 [3]盘锦市双台子河口自然保护区,盘锦124000 [4]School of Pharmacy

出　　处：《Acta Genetica Sinica》2003年第5期419-424,共6页

基　　金：国家自然科学基金资助项目 (编号 :39970 389)~~

摘　　要：采用PCR直接测序方法首次对形目 (Charadriiformes) 12种鸟类 :蒙古沙 (Charadriusmongolus)、环颈(Charadriusalexandrinus)、大杓鹬 (Numeniusmadagascariensis)、白腰杓鹬 (Numeniusarquata)、中杓鹬 (Numeniusphaeo pus)、红脚鹬 (Tringatotanus)、林鹬 (Tringaglareola)、翘嘴鹬 (Xenuscineres)、翻石鹬 (Arenariainterpres)、大滨鹬 (Calidristenuirostris)、反嘴鹬 (Recurvirostraavosetts)和砺鹬 (Haematopusostralensis)线粒体cytb基因全序列进行测定 ,并以白鹳(Ciconiaciconia)的同源序列作为外群构建系统发生树。经比对 ,形目 12种鸟类线粒体cytb基因全序列均包括1143bp ,序列间未见有插入和缺失 ,共有 381个变异位点 ,种间序列差异值为 5 16 %～ 16 0 1%。重建的系统树将形目 12种鸟类分为 2个支系 :第 1支系包括红脚鹬、林鹬、翻石鹬、大滨鹬、翘嘴鹬、中杓鹬、大杓鹬和白腰杓鹬 ,其中红脚鹬、林鹬、翻石鹬、大滨鹬、翘嘴鹬聚为一支 ,中杓鹬、大杓鹬和白腰杓鹬聚为另一支 ;第 2支系包括蒙古沙、环颈、反嘴鹬和砺鹬 ,其中反嘴鹬与砺鹬互为姐妹群 ,然后再与属的两个种蒙古沙和环颈组成的姐妹群构成并系群。分子证据提示 :第 1支系中各属间及种间的系统关系与形态学研?Studies of the phylogenetic relationships of the Charadriiformes have been largely based on conservative morphological characters.During the past 10 years,many studies on the evolutionary biology of birds adopted phylogenetic information obtained from mitochondrial DNA,but few work on the Charadriiformes has been reported to date.Therefore,phylogenetic relationships and classification of the Charadriiformes remains controversial.In this study,we try to shed light on these relationships via DNA sequence analysis of the mitochondrial Cyt b gene in 12 species of Charadriiformes.It was a preliminary study of the origin and evolution of the species by using nucleotide sequence data.Using the well-known PCR techniques,the complete mitochondrial Cyt b gene sequences were amplified and sequenced respectively from Charadrius mongolus,Charadrius alexandrinus,Numenius madagascariensis,Numenius arquat,Numenius phaeopus,Tringa totanus,Tringa glareola,Xenus cineres,Arenaria interpres,Calidris tenuirostris,Recurvirostra avosetts and Haematopus ostralensis.The 1143 bp long DNA sequences of the gene from these species were obtained,in which 381 variable sites were identified without insertions or deletions.The nucleic acid sequence variation of the mitochondrial Cyt b gene was 5.16%～16.01% among these species.Phylogenetic trees constructed using the NJ method,MP method and ML mothod with Ciconia ciconia as the outgroup indicate that the 12 species of Charadriiformes examined in this study are clustered in two major clades.The first clade includes T.totanus,T.glareola,A.interpres,C.tenuirostris,X.cineres,N.madagascariensis,N.arquata and N.phaeopus.The second one includes C.mongolus,C.alexandrinus,R.avosetts and H.ostralensis.Our molecular data show that the phylogenetic relationships among species of Scolopacidae are consistent with the classification based on morphological studies;R.avosetts and H.ostralensis are relatively closer,and form a sister group,and then form paraphyletic group with a sister group which comprised of C.

关 键 词：Hang形目 CYTB基因 DNA序列 系统进化 

分 类 号：Q953[生物学—动物学]