脂质运载蛋白型前列腺素D合酶在脑胶质瘤中的表达研究  

Expression of lipocalin-type prostaglandin D synthase in glioma

作　　者：苏兵[1] 关明[1] 刘维薇[1] 吕元[1] 

机构地区：[1]复旦大学附属华山医院检验医学中心,上海200040

出　　处：《中华检验医学杂志》2003年第3期162-165,共4页Chinese Journal of Laboratory Medicine

摘　　要：目的　研究脂质运载蛋白型前列腺素D合酶 (L PGDS)mRNA及其蛋白在脑胶质瘤中的表达。方法　用实时荧光定量逆转录聚合酶链反应 (RT PCR)和免疫印记 (westernblot)检测L PGDSmRNA及其蛋白在脑胶质瘤组织和脑脊液中的表达水平。结果　实时荧光定量RT PCR显示 ,L PGDS的mRNA在 2 3例脑胶质瘤组织中的表达水平范围为 1 5× 10 3～ 7 0× 10 4 copy/μgRNA ,均值为 1 6×10 4 copy/μgRNA ,而在 5名正常组织中的表达水平均值为 8 1× 10 5copy/μgRNA。经统计学分析 ,两组间差异有显著性 (P <0 0 5 ) ,且表达水平与胶质瘤恶性程度相关。胶质瘤细胞株U 2 5 1的表达水平为4 7× 10 3copy/μgRNA。蛋白定量显示 ,脑胶质瘤患者脑脊液中L PGDS的吸光度比率均值为 0 47±0 12 ,而正常脑脊液中为 0 92± 0 2 6 ,两组间差异有非常显著性 (P <0 0 1)。结论　发现在脑胶质瘤中L PGDSmRNA及其蛋白表达水平下降 ,这可能对脑胶质瘤的诊断、治疗有潜在的应用价值。Objective To investigate the expression level of lipocalin-type prostaglandin D synthase (L-PGDS) mRNA and its protein in glioma.Methods The L-PGDS mRNA was determined by real-time quantitative RT-PCR in 23 glioma and 5 healthy brain specimens. L-PGDS protein was detected by Western blot in cerebrospinal fluid (CSF) and brain tissue specimens.Results In 23 glioma specimens L-PGDS mRNA expression ranges from 1.5×10 3～7.0×10 4 copy/μg RNA, mean value is 1.6×10 4 copy/μg RNA. In 5 normal brain specimens, the mean value is 8.1×10 5 copy/μg RNA, statistic analysis indicates a distinct discrepancy ( P<0.05), and L-PGDS mRNA expression level correlates with the histological grade of malignancy. For glioma cell line U-251 the expression level of L-PGDS mRNA is 4.7×10 3 copy/μg RNA. Western blot indicates that L-PGDS protein expression level is 0.47±0.12 in glioma and 0.92±0.26 in healthy people, statistic analysis indicates a distinct discrepancy ( P<0.01).Conclusions It is found that the expression level of L-PGDS mRNA and its protein decrease in glioma. This implies that L-PGDS has a potential value for diagnosis and therapy of glioma.

关 键 词：脂质运载蛋白型前列腺素D合酶 脑胶质瘤 荧光定量逆转录聚合酶链反应 免疫印记 

分 类 号：R739.4[医药卫生—肿瘤]