用胚胎干细胞诱导的造血干/祖细胞重建小鼠造血功能研究  

Reconstitute of the Hematopoietic Function of Mice Using Hematopoietic Stem/Progenitor Cells Derived from Embryonic Stem Cells

作　　者：周其锋[1] 何志旭[2] 冯炼强[1] 黄绍良[2] 李树浓[3] 

机构地区：[1]中山大学免疫学教研室 [2]中山大学孙逸仙纪念医院儿科 [3]中山大学病理生理学教研室,广州510080

出　　处：《热带医学杂志》2003年第1期9-11,共3页Journal of Tropical Medicine

基　　金：国家自然科学基金资助项目(No.39970319)

摘　　要：目的胚胎干细胞体外诱导分化出CD34+造血干/祖细胞,然后将其注射入致死量照射小鼠体内,以研究其重建造血功能。方法胚胎干细胞自由分化形成胚胎体后,加入造血刺激因子混合液以诱导产生CD34+造血干/祖细胞,将这些造血干/祖细胞注射入经致死剂量照射的小鼠,观察小鼠存活率的改变,并取存活2个月的小鼠骨髓和脾脏,PCR检测嵌合体形成。结果体外分化第13日CD34+造血干/祖细胞比例可高达17.36％,将这些细胞注射入致死量照射小鼠后,可提高存活率达86.67％,存活小鼠的骨髓和脾脏均检测到供体来源的细胞。结论胚胎干细胞体外分化获得CD34+造血干/祖细胞,且后者具有其相应的正常生物学功能。Objective To induce Embryonic stem cells to differentiate into CD34+ hematopoietic stem/progenitor cells in vitro. To reconstitute the hematopoietic function of lethally irradiated mice. Methods Embryonic stem cells, formed embryoid bodies, were induced to differentiate into CD34+ hematopoietic stem/progenitor cells when cultured with hematopoietic growth factors. These CD34+ hematopoietic stem/progenitor cells were injected into lethally irradiated mice. PCR was performed to detect the sex-determining region of the Y-chromsome derived from the male donor cells in the bone marrow and the spleen cells of the transplanted female mice. Results The percentage of CD34+ cells could reach 17.36% on the thirteenth day. The survival rate of the group transplanted with CD34+ hematopoietic stem/progenitor cells was 86.67% . The donor cells were both found in the bone marrow and the spleen cells of the survived transplanted mice. Conclusion Embryonic stem cell could be induced to differentiate into CD34+ hematopoietic stem/progenitor cells in vitro, and these cells could reconstitute the hematopoietic function of the lethally irradiated mice.

关 键 词：胚胎干细胞 诱导 造血干/祖细胞 重建 小鼠 造血功能 研究 

分 类 号：R321[医药卫生—人体解剖和组织胚胎学] R329.1[医药卫生—基础医学]