抗日本血吸虫病分子疫苗(SIEA28kDa)氨基酸组成的分析  

Analyzing of the Amino Acid of the 28 kDa SIEA of Schistosoma japonicum

作　　者：谭耀武[1] 汪世平[1] 刘立鹏[1] 徐绍锐[1] 

机构地区：[1]中南大学湘雅医学院,长沙411300

出　　处：《热带医学杂志》2003年第1期32-34,共3页Journal of Tropical Medicine

基　　金：国家"十.五"重大专项资助课题(No.2002AAZ3343);国家自然科学基金资助项目(No.39870652);湖南省重点学科建设项目联合资助

摘　　要：目的采用反向高效液相色谱方法分析SIEA28kDa蛋白分子的氨基酸组成。方法用邻苯二甲醛(OPA)柱前衍生反相高效液相色谱法测定SIEA28kDa抗原组分样品中氨基酸成分,并在Expasy中数据分析与比较。结果SIEA28kDa分子抗原主要由13种氨基酸组成。其中,甘氨酸在其氨基酸组成中比值最大,在Expasy中根据其氨基酸组成的比值及其一级属性参数比较发现6种Score值较高的血吸虫蛋白质分子,但SIEA28kDa分子与它们均有不同方面的差别。结论SIEA28kDa分子可能不是6种Score值较高的血吸虫蛋白质分子中的任何1种,但与它们在氨基酸组成上具有较高的相似性,而与GST相似性较低。Objective To analyze the amino acids composition of the 28kDa molecular derived from immature egg antigen (SIEA) of Schistosoma japordcum. Methods Amino acids composition of the 28kDaSIEA were determined by precolumn derivatization with 0-phthaldialdehyde ( OPA) and reversed-phase high performance liquid chromatography. The amino acid composition was analyzed in Expasy web site. Results 28kDaSFEA was mainly composed of !3 amino acids,in wliich glycine was ihe most abundant amino acid. By comparing in Expasy, it was found that theie were six schistosome proteins with score more than 2500, but showed some difference with the 28kDaSIEA. Conclusion The 28XkDaSIEA may be not be one of the six schistosome proteins showing high score in comparision,and has low similarity with GST in amino acid constitution.

关 键 词：抗日本血吸虫病分子疫苗 氨基酸 分析 未成熟虫卵 抗原 高效液相色谱 

分 类 号：R383.24[医药卫生—医学寄生虫学]