火菇素抗体探针的制备与高灵敏度的火菇素定性技术的建立  

Preparation of Rabbit Antibody Probe and Qualitative Analysis Specific for Flammulin

作　　者：周凯松[1] 常宁[1] 彭俊峰[1] 张晗星[1] 陈畅[1] 张长铠[1] 

机构地区：[1]山东大学微生物技术国家重点实验室,济南250100

出　　处：《山东大学学报（理学版）》2003年第1期117-120,共4页Journal of Shandong University(Natural Science)

基　　金：教育部博士点基金 (No .2 0 0 0 0 42 2 12 )

摘　　要：从金针菇子实体浸提液中提取了抗癌活性物质火菇素 ,将纯化的火菇素用Freund佐剂乳化后注射到新西兰白兔体内 ,经数次加强免疫后采血并分离了抗血清 ,应用免疫学方法检测与硝酸纤维素滤膜上抗原蛋白相结合的抗体探针 ,检测极限可达 2 0 - 4Flammulin, an anti tumor protein, was purified to electrophoretic homogeneity from the aqueous extract of basidiomes of Flammulina Velutipes. Purified flammulin emulsified with Freund's adjuvant was injected subcutaneously into New Zealand white rabbits. After several immune enhancements, these animals were bled and sera were separated. Western blotting had be used to detect antibody bound to its antigen on nitrocellulose filters, 20-40pg of antigen could be detected.

关 键 词：火菇素 金针菇 抗体探针 定性分析 

分 类 号：Q51[生物学—生物化学]