肺癌化疗前后p53表达和细胞凋亡的变化  

Clinical Significance of the Expression of Apoptosis and p53 in Lung Cancer

作　　者：吴山[1] 盛延良[2] 蒋志学[2] 孙明[2] 

机构地区：[1]重庆医科大学临床学院外科 [2]佳木斯大学

出　　处：《中国医师杂志》2003年第5期589-591,共3页Journal of Chinese Physician

摘　　要：目的　探讨化疗对肺癌细胞凋亡、p5 3蛋白表达的影响。方法　收集实验组 14例肺癌病人化疗前后标本和对照组术前未作化疗肺癌标本 2 8例 ,采用免疫组化法检测p5 3蛋白表达 ,采用TUNEL法检测细胞凋亡 ,并作化疗前后的比较。结果　对照组和实验组在化疗前后p5 3阳性表达无显著差异 (P >0 0 5 ) ,而化疗后凋亡指数明显升高 (P <0 0 1)。结论　化疗可诱发肺癌细胞凋亡 ,但对p5Objective To determine the effects of pre-operative chemotherapy on p53 expression and apoptosis in lung cancer.Methods 42 patients with lung cancer were divided into pre-opreative chemotherapy group (n=14) and control group (n=28) without preoperative chemotherapy.Method of immunohistochemistry was used to detect the expression of p53 . Terminal deoxynucleotidyl transferase nick end labeling (TUNEL) was used to detect the apoptotic cells.Results The positive rate of p53 expression were as follows;53 5% for control group,64 3% for prechemotherapy group,64 3% for postchemotherapy group respectively;the apoptosis indexes were 6 3±1 9 for control group,7 3±1 6 for prechemotherapy group,14 5±4 6 for postchemotherapy group.Conclusions The preoperative chemotherapy can induce apoptosis in lung cancer ,but can not affect p53 expression .

关 键 词：肺癌 化疗 凋亡 P53 

分 类 号：R734.2[医药卫生—肿瘤]