PCR直接检测龈下菌斑主要可疑牙周致病菌  

DETECTION OF MAIN PUTATIVE PERIODONTOPATHIC BACTERIA IN SUBGINGIVAL PLAQUE BY POLYMERASE CHAIN REACTION

作　　者：王者玲[1] 岗本公彰 杨圣辉[1] 前田伸子 李金陆[1] 母瑞红[1] 刘颍[1] 

机构地区：[1]首都医科大学口腔医学院研究所,北京100050 [2]日本鹤见大学齿学部

出　　处：《中国微生态学杂志》2003年第2期115-116,共2页Chinese Journal of Microecology

摘　　要：目的 :应用 PCR方法直接检测龈下菌斑主要可疑牙周致病菌与牙周病活动部位的关系 ,探讨其方法的可行性并探讨其主要可疑牙周致病菌的分布规律。方法 :应用聚合酶链反应 ( polymerase chainreaction,PCR)直接检测龈下菌斑主要可疑致病菌 16 s RNA保守区域片段。 4 0名受试者包括牙周病患者2 0人 ,每人同口取一个牙周病活动部位 ,一个相对健康或牙周病静止对照部位 ;成人健康者 2 0人 ,每人各取一个标本。结果 :龈下菌斑 5种可疑牙周致病菌在牙周病活动部位的检出率牙龈卟啉菌为 86 % ,福赛类杆菌为 95 % ,螺旋体为 86 % ,中间普氏菌和黑色普氏菌分别为 95 %和 33% ,均显著高于同口部位对照组和健康对照组。结论 :PCR直接检测菌斑牙龈卟啉单胞菌、中间普氏菌、福赛类杆菌。Objectives:To investigate the possibility of detection of five major putative periodontopathic bacteria subgingival plaque using the polymerase chain reaction(PCR)method was in periodontal patients and healthy subjects Methods:The 16S rRNA gene directed PCR method was used to detect the putative periodontopathic bacteria in subgingival plaque samples A total of 20 periodotitis patients and 20 healthy subjects were undergone in this study Subgingival plaque was taken from one active site and one healthy site in each periodontal diseases patient,and one site from healthy subject Results:The prevalence of Porphyromonas gingivalis,Prevatella intermedia,Bacterodies forsythus,Treponema denticola and Prevotella nigrescens in active sites of periodontal patients were 86%,95%,95% 86% and 33%,respectively,which were markly higher than the sites in the same oral cavity and healthy subjects sites of the control groupes Conclusion:The five putative periodontopathic bacteria were associated with active sites of periodontitis patients and the PCR might be useful for the detection of periodontopathic bacteria from subgingival plaque directly

关 键 词：牙周致病菌 龈下菌斑 PCR 直接检测 牙周病活动部位 分布规律 

分 类 号：R781.4[医药卫生—口腔医学]