原废水培养基分离活性污泥中的苯酚降解细菌  

ISOLATION OF NOVEL PHENOL-DEGRADING BACTERIA FROM ACTIVATED SLUDGE USING FEED WATER MEDIUM (FWM)

作　　者：高平平[1] 陈迎春[1] 刘彬彬[1] 张学礼[1] 赵立平[1] 

机构地区：[1]上海交通大学生物工程系

出　　处：《应用与环境生物学报》2003年第2期189-192,共4页Chinese Journal of Applied and Environmental Biology

基　　金：国家高科技研究发展计划项目资助("863"项目计划)~~

摘　　要：用未经处理的含酚焦化工业废水为基础配制培养基,采用平板涂布法,在10^(-5)稀释度下得到100个单菌落分离物.它们均能在以苯酚(500～800 mg L-1)为唯一碳源的无机盐培养基上生长,其16S rDNA基因的ARDRA多态性分析将这些分离物划分成4个分类操作单元(OTUs).各单元代表株的16S rRNA基因序列分析和检索结果表明,97个分离物与Alcaligenes faecalis同源性达99％,2个分离物分别与Arthrobacter nicotianae和Klebsiella sp.99％同源,另一个分离物与Ochrobactrum sp.96％同源.苯酚羟化酶大亚基基因(LmPHs)PCR扩增表明,除类似Arthrobacter nicotianae的菌株外,其它3个分类操作单元的降酚菌都能利用多组分苯酚羟化酶代谢途径进行酚代谢.100 isolates were obtained from feed water solid media using spread-plate method with diluted activated sludge ( 10 -5 dilution rate). All the isolates were capable of growing on a synthetic medium with phenol (500 ~ 800 mg L -1 ) as a sole carbon source and classified into 4 operational taxoriomic units (OTUs) by amplified ribosomal DNA restriction analysis (ARDRA). The full-length 16S rDNA gene of one representative strain for each OTU was amplified, cloned, sequenced and blasted against the GenBank database. The results showed that 97 isolates were closely related to Alcaligenes faecalis with 99% homology; two isolates showed 99% identity to the sequence of Arthrobacter nicotianae and Klebsiella sp. respectively; and the last one exhibited 96% homology with Ochrobactrum sp. The specific amplification of the gene encoding the large subunit of the multi-component phenol hydroxylase (LmPHs) revealed that three out of four OTUs employed the metabolic pathway catalyzed by multicomponent phenol hydroxylase to degrade phenol, while the specific amplification from the Arthrobacter nicotianae-like strain did not show any products, indicating a possibly different phenol-degrading pathway. Fig 2, Tab 2, Ref 11

关 键 词：苯酚 生物降解 降解细菌 焦化工业废水 培养基 活性污泥 分离 筛选 

分 类 号：X172[环境科学与工程—环境科学]