失巢凋亡过程中整合蛋白α5β1过表达对人肝癌细胞Survivin表达的影响  

Effect of Over -expression of Integrin α5β1 Gene on Expression of Survivin in Human Hepatocarcinoma during Anoikis

作　　者：雷霆雯[1] 梁玉龙[2] 查锡良[2] 

机构地区：[1]贵阳医学院生物化学与分子生物学教研室,贵州贵阳550004 [2]复旦大学医学院生化教研室,上海200032

出　　处：《贵阳医学院学报》2003年第2期137-139,共3页Journal of Guiyang Medical College

摘　　要：目的:探讨整合蛋白α5β1过表达引起肝癌细胞凋亡的机理。方法:利用poly-hema使细胞非贴壁生长12 h、18 h、24 h,诱导失巢凋亡。通过Western印迹法检测Survivin表达量。结果:随着Poly-hema诱导时间的增加,α5β1-7721和β1-7721细胞凋亡和死亡率也随之增加而Survivin表达量则减少,β1-7721细胞的减少程度显著大于α5β1-7721,在18h时减少了55％。结论:Survivin在β1-7721细胞的凋亡过程中所起的作用大于α5β1-7721细胞。Objective: To study the causing mechanism of over - expression of integrin α5β1 to anoikis in hepatocellular carcinoma cells. Methods: Poly-hema was used to let cells grow freely from anchorage for 12h, 18h, and 24h to induce anoikis. Western blot assay was adopted to examine the expression level of survivin. Results: The rates of apoptosis and death increased with times in strains of α5β1 -7721 and β1-7721, but survivin expression decreased. Survivin expression was significantly lower in strain β1-7721 than that in α5β1-7721. Conclusion: Survivin might play an important role in anoikis of α5β1-7721 and β1-7721 cells, especially of β1-7721.

关 键 词：失巢凋亡 整合蛋白α5β1 肝癌细胞 SURVIVIN表达 影响 细胞凋亡 基因表达 

分 类 号：R735.7[医药卫生—肿瘤]