玉米小斑病菌C小种毒素对玉米雄性不育系及其保持系超氧物歧化酶及过氧化物酶活性的诱导  

Effect of Bipolaris (Helminthosporium) Maydis Race C (HMC) Toxin on Superoxide Dismutase and Peroxidase Activities of Corn with Male Sterile or Normal Cytoplasms

作　　者：崔洋[1] 马春红[1] 刘克明[1] 魏建昆[1] 

机构地区：[1]河北省农林科学院农业物理生理生化研究所,石家庄050051

出　　处：《华北农学报》1992年第1期25-30,共6页Acta Agriculturae Boreali-Sinica

基　　金：国家自然科学基金资助课题

摘　　要：对玉米雄性不育系及保持系B73叶片超氧化物歧化酶(SOD)活性的测定表明,细胞溶质最高,玉米雄性不育系B73-C,B73-T及其保持系B73-N叶片基态超氧物歧化酶活性在细胞内的分布是:细胞溶质最高(占总活性77％);线粒体、叶绿体次之,且SOD活性N细胞质显著大于C和T细胞质;过氧化物酶(POX)的活性主要分布在线粒体和叶绿体中,占总活性85％左右。用专化于C细胞质的C毒素(HMC毒素)处理B73C、T与N三种细胞质玉米叶片后,这三种细胞溶质的SOD和POX酶活性均表现不敏感;三种细胞质的线粒体有不同反应:B73-C的SOD活性下降,导致细胞伤害,而B73-T和B73-N的SOD活性稍有提高或持平,保持了对C毒素的抗性。POX基本上随着SOD活性降低而增高或随着活性增高而降低。可见,HMC毒素能够诱导线粒体SOD和POX的活性,而这两个酶活性与玉米抗病性密切相关。The localization of superoxide dismutase (SOD) and peroxidase(POX) activities in leaf cells of corn with male sterile cytoplasm (B73-C B73-T)and normal cytoplasm (B73-N)was determined.The results showed that SOD activity was the highest in cell solute (about 77% of the total) and lower in mitochondria and chloroplast.SOD activity of cell solute and mitochondria in N cytoplasm was significantly higher than that in C and T cytoplasms .As to POX, about 85% of its total activity was present in the mitochondria and chloroplast, and 15% was in the cell solute. When the leaves of all the three cytoplasms (B73-C, T and N) were treated with the host-specific toxin produced by B. maydis race C, their SOD and POX activities in the cell solutes were not affected. There existed a negative correlation between SOD and POX activities in mitochondria of B73-C, B73-T and B73-N. Therefore, the activities of SOD and POX in mitochondria could be induced by HMC-toxin and the activity of both enzymes was related to disease resistance, of corn.

关 键 词：玉米 小斑病菌 雄性不育 酶活性 

分 类 号：S513.034[农业科学—作物学]