藻胆蛋白脱辅基蛋白对其抗氧化活性的影响  

EFFECT OF APOPROTEIN ON ANTIOXIDANT ACTIVITY OF PHYCOBILIPROTEINS

作　　者：周站平[1] 陈秀兰[1] 陈超[1] 张玉忠[1] 

机构地区：[1]山东大学微生物技术国家重点实验室,济南250100

出　　处：《海洋科学》2003年第5期77-80,F003,共5页Marine Sciences

基　　金：国家863计划课题2002AA302213号;山东省自然科学基金

摘　　要：探讨了光照和脱辅基蛋白结构对藻胆蛋白抗氧化活性的影响,结果表明,光照下,藻胆蛋白能够产生自由基,黑暗下,则能够清除自由基,因此,藻胆蛋白具有产生和清除自由基的双重功能。在光照下,用SDS(十二烷基硫酸钠)、脲及碱性条件下,对藻胆蛋白进行变性,藻胆蛋白产生自由基的能力消失,清除自由基的能力明显增强,因此,藻胆蛋白清除自由基主要由藻胆色素完成,天然状态下藻胆蛋白中脱辅基蛋白三维结构更有利于色素基因对光能的吸收与传递,这与其在体内的生理功能相一致。This article studied the effects of light and apoproteins' structure on antioxidant activity of Phycobiliproteins. The results showed that Phycobiliproteins could generate hydroxyl radicals in light and scavenge them in dark. Its abilities of generating radical disappeared and scavenging radical greatly increased when Phycobiliproteins were denatured by SDS, urea and in alkaline condition. The results showed that the Phycobilins were the main part of Phycobiliproteins to scavenge hydroxyl radical. The Apoproteins' structure of nature Phycobiliproteins was at most fit for absorption and transfer of light, which was consistent with the function of Phycobiliproteins in Spirulina platensis.

关 键 词：藻胆蛋白 脱辅基蛋白 光照 抗氧化活性 捕光色素蛋白 

分 类 号：Q946.1[生物学—植物学]