高剂量率^(60)Coγ射线照射离体人血建立染色体畸变的剂量-效应曲线  

Established Curves of Relationships between Radiation Dose and Chromosome Aberra tions Induced by High Dose Rate ^(60)Co γ Ray in Human Perip heral Blood Lymphocytes in Vitro

作　　者：白玉书[1] 黄绮龙[1] 马剑锋[1] 关树荣[1] 雷苏文[1] 李文建[2] 苏旭[1] 

机构地区：[1]中国疾病预防控制中心辐射防护与核安全医学所,北京100088 [2]中国科学院兰州近代物理研究所

出　　处：《中国预防医学杂志》2003年第2期100-103,共4页Chinese Preventive Medicine

摘　　要：目的　高剂量率 6 0Coγ射线照射离体人血建立染色体畸变的剂量 -效应曲线。方法　6 0 Coγ射线照射离体人血 ,吸收剂量为 1 0～ 8 0Gy ,剂量率为 3 0Gy/min ,染色体采用微量法 ,培养开始加秋水仙碱 ,主要记录染色体型畸变的非稳定性畸变 ,包括无着丝粒断片、微小体、无着丝粒环、双着丝粒体和着丝粒环 (双 +环 ) ,对实验数据做曲线拟合 ,并检验回归系数的显著性和曲线的拟合度。结果　6 0 Coγ射线照射离体血诱发的“双 +环” ,在 0～8 0Gy范围内 ,呈良好的剂量 -效应关系 ,拟合的最佳回归方程为Y =0 2 69792D + 7 73 0 83× 10 - 2 D2 (R =0 99198)。结论　高剂量率 6 0 Coγ射线照射离体人血 ,采用微量法培养染色体 ,在 0～ 8 0Gy范围内 ,“双 +环”最佳数学模式为Y =bD +cD2 。Objective To establish curve s of relationships between radiation dose and chromosome aberrations induced by high dose rate 60 Co γ-ray in human peripheral blood lymph oc ytes in vitro Methods Heparinized who le b lood samples were exposed to 60 Co γ-ray Radiation doses wer e from 1 0 Gy to 8 0 Gy Dose rate was 3 0 Gy/min Microculture method was ap plied with culture beginning to add colchicine Unstable chromosome aberrations inclu d ing acentric fragments,minutes,acentric rings,dicentrics and cenric rings('d ic+ r')were scored Results Chromosome aberra tions('dic+r')induced by 60 Co γ-ray had a good dose-res po nse relationships Fitting the best regression equation was Y=0 269792D +7 73083×10 -2 D 2(R=0 99198) Con cl usion The best mathematical model fitting of chromosome aberra tions('dic+r')induced by high dose rate 60 Co γ-ray was Y=bD+cD 2

关 键 词：高剂量率 ^60Coγ射线 照射 离体人血 染色体畸变 剂量-效应曲线 

分 类 号：R818.03[医药卫生—放射医学]