蛋白酶水解铬革屑所得胶原蛋白产物分子量的研究  

Study on the MW of collagen hydrolyzed from chrome shavings catalysed by protease

作　　者：李天铎[1] 崔凤霞[1] 侯同刚[1] 李俊英[1] 张庆思[1] 刘素清 

机构地区：[1]山东轻工业学院,济南250100 [2]山东省皮革工业研究所,济南250021

出　　处：《中国皮革》2003年第5期4-5,9,共3页China Leather

摘　　要：用经典凝胶柱层析和凝胶电泳相结合的方法 ,对蛋白酶水解铬革屑所得胶原蛋白产物进行了分子量测定 ,并用高效毛细管电泳法进行对比验证。研究表明 :在本文建立的水解条件下 ,水解产物中分子量为5 0 0 0 0 - 70 0 0 0 D之间的占 10 .7%,2 0 0 0 0 - 30 0 0 0 D之间的占 89.3%。The molecular weight (MW) of the collagen hydrolyzed from chrome shavings catalysed by protease was determined through classical gel filtration chromatography (GFC) combined with sodium dodecyl sulpate-polyacrylamide gel electrophoresis (SDS-PAGE). Furthermore, the effects were contrasted by high performance capillary electrophoresis(HPCE). The study showed that in the products peptides with MW between 50 000 to 70 000D were 10.7% , between 20 000 to 30 000D were 89.3%.

关 键 词：蛋白酶水解 铬革屑 胶原蛋白产物 分子量 凝胶柱层析法 凝胶电泳法 高效毛细管电泳法 测定 

分 类 号：TQ93[化学工程]