烟草和元麦叶肉原生质体吸收尼古丁机制的研究  

Study on Mechanism of Nicotine Uptake by Nicotiana tabacum and Hordeum distichon Mesophyll Protoplasts

作　　者：黄祥辉[1] 虞乃而[1] 颜季琼[1] 

机构地区：[1]华东师范大学生物学系

出　　处：《华东师范大学学报（自然科学版）》1992年第1期101-106,共6页Journal of East China Normal University(Natural Science)

基　　金：国家自然科学基金

摘　　要：用 EA_3-867纤维素酶分离的烟草(Nicotiana tabacum)和元麦(Hordeum distichon)叶肉原生质体,分别保温在尼古丁溶液中,用紫外分光光度计测定它们对尼古丁的吸收。结果表明,这两种原生质体能迅速地吸收尼古丁;原生质体吸收尼古丁的量,随介质尼古丁浓度的升高而增多;在生理学pH范围内,介质的pH对原生质体吸收尼古丁没有多大的影响。初步认为,这两种原生质体对尼古丁的吸收是一种简单的扩散机制。Mesophyll protoplasts of the Nicotiana tabacum and Hordeum distichon releasedby EA_3-867 cellulase, respectively were incubated in medium containing nicotine.Nicotine uptake by the protoplasts was measured with Beckman DU-7 spectropho-tometer. The results indicates that the nicotine was rapidly uptaked by the twospecies protoplasts; The quantity of the nicotine uptake by the protoplasts increasedalong with raise of the nicotine concentration; In physiological pH range, theeffect of pH on the nicotine uptake by the protoplass was small. In light of the abo-ve findings, the preliminary assumption is that the nicotine uptake mechanism bytwo species protoplasms is a simple diffusion.

关 键 词：尼古丁 原生质体 扩散 元麦 吸收 

分 类 号：Q946.881[生物学—植物学]