纳他霉素高产菌株的选育及其发酵条件的研究  

Study on the Breeding of High Natamycin Producing Strains and Their Fermentation Conditions

作　　者：陈冠群[1] 杨东靖[1] 杜连祥[1] 

机构地区：[1]天津科技大学食品科学与生物工程学院

出　　处：《食品与发酵工业》2003年第3期19-22,共4页Food and Fermentation Industries

基　　金：天津市自然科学基金资助项目 (No.0 136 0 9511)

摘　　要：利用链霉素抗性突变选育纳他霉素高产菌株 ,即将经过紫外线诱变处理的纳他霉素(natamycin)产生菌褐黄孢链霉菌 (Streptomycesgilvosporeus)ATCC1 332 6菌株孢子涂布在含有链霉素最小抑制浓度 (0 6μg/mL)的培养基平板上 ,获得了 1 2 2株链霉素抗性突变株。其中纳他霉素产量高于出发菌株的有 1 3株。其中突变株SG 56的生产能力比出发菌株提高到 1 4 6 % ,研究了其发酵性能 ,优化了培养基组成和发酵工艺条件 ,使纳他霉素产量提高到 1 70 %。A method of streptomycin resistance screening was applied to breed mutants with high natamycin productivity The spores of Streptomyces gilvosporeus(ATCC13326) treated with UV light were regenerated on agar plates containing 0 6*#μg/mL streptomycin 122 streptomycin resistant mutants were obtained The natamycin yields of 13 mutants were higher than that of the original strain Among them, the yield of SG 56 was 46% more than the original strains The medium and fermentation conditions of SG 56 were optimized Under the optimum condition, the natamycin production increased by 70% in comparison with that of the parent one

关 键 词：纳他霉素高产菌株 选育 发酵条件 链霉素抗性突变 诱变育种 褐黄孢链霉菌 食品防腐剂 

分 类 号：TQ465[化学工程—制药化工]