中空纤维膜过滤法高密度培养凝结芽孢杆菌TQ33  

High-density Cultivation of Bacillus coagulans TQ33 with Hollow Fiber Filtration

作　　者：戚薇[1] 刘馨磊[1] 王建玲[1] 陈莹[1] 路福平[1] 杜连祥[1] 

机构地区：[1]天津科技大学食品与生物工程学院

出　　处：《食品与发酵工业》2003年第4期15-18,共4页Food and Fermentation Industries

基　　金：天津市自然科学基金重点研究资助项目 (No 0 0 380 361 1 );天津市高等学校科技发展基金资助项目 (No 990 90 3)

摘　　要：利用中空纤维超滤装置进行TQ3 3高密度培养 ,确定了采用过滤法进行高密度培养的工艺流程和参数。在对数后期 ,当乳酸质量浓度达到 15g/L时 ,开始过滤培养。过滤培养阶段持续 6h ,培养过程中平均过滤速率和培养基的流入速率为 1L/h ,平均稀释率为 0 4/h。对通过试验所确定的工艺进行高密度培养 ,最终菌体和芽孢浓度分别达到 1 6× 10 10 cfu/mL和 1 2× 10 10 cfu/mL ,是分批培养的 2 1倍和 3The high density cultivation of Bacillus coagulans TQ33 were carried out in a 5L fermentor equipped with a hollow fiber filter module The cultivation processing and operation parameters were determined After lactic acid concentration reached 15?g/L at the late stage of log phase,biomass feeding and the filtration cultivation was continued for 6 hours The filtering rate as well as the feed rate of fresh medium was around 1?L/h,and the dilution rate was 0 4/h The cell concentration was 1 6×10 10 ?cfu/mL,and the spore concentration was 1 2×10 10 ?cfu/mL,which was 21 times and 37 times as that of the batch culture,respectively

关 键 词：过滤法 高密度培养方法 凝结芽孢杆菌 中空纤维超滤装置 

分 类 号：TS252[轻工技术与工程—农产品加工及贮藏工程]