胰岛素对CWE纯系小鼠脑组织细胞癌基因表达的影响  

Effects of Insulin on Expression of Oncogenes in CWE Mouse Brain

作　　者：孙宁[1] 孙芝琳[1] 

机构地区：[1]华西医科大学生物化学教研室

出　　处：《华西医科大学学报》1992年第2期164-167,共4页Journal of West China University of Medical Sciences

基　　金：国家自然科学基金

摘　　要：作者给正常GWE小鼠皮下注射胰岛素后,利用斑点杂交法观察小鼠脑组织中c-myc、c-fos、Ha-ras、erb B基因的表达情况。结果表明:注射胰岛素后小鼠脑中c-myc、c-fos基因的表达量增加,于注射后2小时达最高峰,以后其表达逐渐下降,直至注射后24小时降至最低;注射胰岛素后小鼠脑中Ha-ras基因的表达似有下降,而erb B基因表达量下降。以上结果提示:c-myc、c-fos、Ha-ras、erb B等细胞癌基因与脑细胞生长调控有一定的关系,其产物可能具有外源性生长刺激信号的细胞内传递作用。By RNA dot blot hybridiza-tion, we observed the expression of c-myc,c-fos, Ha-ras and erbB genes in normalbrain of CWE mouse after the injection ofinsulin. Transcription of the c-myc andc-fos genes is greatly increased, whichpeaks approximately 2h after insulin stimu-lation, decreases in 6h and returns to theinitial levels within 48h. The alteration inHa-ras transcription appears more complex;its expression decreases first but increasessubsequently. We also detected the expres-sion of erbB gene which decreases after theinjection of insulin. The results suggest that c-myc, c-fos,Ha-ras and erbB might be directlyinvolved in growth control. Their protienproducts might play a role in intracellularsignal transduction.

关 键 词：胰岛素 脑组织 癌基因 

分 类 号：R730.21[医药卫生—肿瘤]