刺梨及部分近缘种形态学性状和RAPD标记分析  

A Comparative Study of RAPD and Morphological Approaches to Characterize Relationships of Rosa roxburghii Tratt.and Its Relatives

作　　者：文晓鹏 庞晓明[1] 邓秀新[1] 

机构地区：[1]华中农业大学作物遗传改良国家重点实验室 [2]贵州大学农学院,贵阳550025

出　　处：《园艺学报》2003年第2期204-206,共3页Acta Horticulturae Sinica

基　　金：国家自然科学基金重点项目 (39830 2 6 0 ) ;贵州省自然科学基金资助项目 (2 0 0 130 31)

摘　　要：通过 36个形态学性状和 2 9个引物RAPD扩增获得的分子标记 ,探索刺梨及部分近缘种的亲缘关系。结果表明 ,普通刺梨所有基因型之间的亲缘关系较近 ,而与其余种类相对较远 ;无籽刺梨与贵州缫丝花的遗传距离较近 ,形态学和RAPD聚类分析都显示很近的亲缘关系 ;尽管两类遗传距离的排序和聚类分析有一定差异 ,但Mantel相关性检测表现出显著相关性 (r=0 .6 12 2 ,P <0 .0 1)。还就无籽刺梨的可能来源进行了讨论。Thirty six morphological traits and RAPD markers amplified with 29 arbitrary primers were used to discriminate the accessions, and to evaluate the relatedness of Rosa roxburghii Tratt.and a sample set of its relatives. Compared with the relatives, considerable close relationships were found among the genotypes of R. roxburghii Tratt. Furthermore, a high relatedness was also obtained between R. sterilis S. D.Shi and R. kweichowensis Yu et Ku. A Mantel correspondence test showed a significant correlation(r=0.6122, P<0.01) between morphological traits and RAPD markers, despite certain discordance existing in the two approaches. In addition, the origination of R. sterilis S. D.Shi was discussed in the paper.

关 键 词：刺梨 近缘种 形态学 RAPD标记分析 Mantel相关性检测 

分 类 号：S665.9[农业科学—果树学]