2014—2015年黑龙江省牡丹江地区犬细小病毒2型分子流行病学调查  

Molecular Epidemiology of Canine Parvovirus-2 in Mudanjiang Area of Heilongjiang Province from 2014 to 2015

作　　者：耿雨菲[1] 王恩雨[1] 滕井胜[2] 李春秋[1] 姚爽[1] 王志慧[1] 王欣宇[1] 苏明俊 魏姗[1] 赵喜文[1] 孙筱璇 边秀东 张立春[1] 孙东波[1] 

机构地区：[1]黑龙江八一农垦大学动物科技学院,黑龙江大庆163319 [2]黑龙江农业经济职业学院,黑龙江牡丹江157041

出　　处：《畜牧与饲料科学》2015年第10期1-3 7,7,共4页Animal Husbandry and Feed Science

基　　金：黑龙江省高校新世纪优秀人才计划(1252-NCET-016)

摘　　要：为了调查黑龙江省牡丹江地区犬细小病毒(CPV-2)流行情况,利用基于CPV-2部分VP2基因(568 bp)PCR的方法,对2014年5月—2015年4月收集的40份腹泻犬粪便拭子进行了检测与分析。结果显示,在40份样品中,20份样品为CPV-2阳性(50%),其中,新CPV-2a(Ser297Ala)型和CPV-2c型分为别占75%(15/20)和25%(5/20);序列分析结果显示,20个CPV-2毒株VP2基因的核苷酸和氨基酸的相似性分别为98.6%~100%和98.2%~100%;进化树分析表明,与其他国家CPV-2参考毒株相比,鉴定的新CPV-2a型毒株和CPV-2c型毒株与中国CPV-2参考毒株形成一个进化群(A),其中鉴定的新CPV-2a型毒株分布于不同的分支中。结果提示,CPV-2在牡丹江地区犬腹泻病例中呈现高感染率。In order to investigate the epidemiology of canine parvovirus-2 (CPV-2) in Mudanjiang area of Heilongjiang Province, a total of 40 stool samples were collected from dogs with diarrhea in Mudanjiang area of Heilongjiang Province from May 2014 to April 2015. The presence of CPV-2 in the samples was determined by PCR amplification of the partial VP2 gene (568 bp). The results revealed that 20 samples (50%) were positive for CPV-2, of which types of new 2a (Ser297Ala) and 2c accounted for 75% (15/20) and 25% (5/20), respectively. Sequence analysis revealed that 20 CPV-2 strains identified in our study showed 98.6%-100% nucleotide identity and 98.2%-100% amino acid identity. Phylogenetic analysis revealed that the identified 20 CPV-2 strains formed a specific group (A) with Chinese reference CPV-2 strains, of which the new 2a strains were divided into different clusters, when compared with CPV-2 reference strains from other countries. These data suggested that CPV-2 showed high prevalence in dogs with diarrhea in Mudanjiang area of Heilongjiang Province.

关 键 词：犬 腹泻 CPV-2 CPV-2a VP2基因 分子流行病学 

分 类 号：S858.292[农业科学—临床兽医学]