鸟苷酸环化酶C及其内生性配体在人胃癌和癌前病变组织中的表达及临床意义  

Expression and clinical significance of guanylyl cyclase C and its endogenous ligands in human gastric cancer and precancerous lesions

作　　者：蒋伟[1] 张健锋[2] 张弘[2] 朱惠君[3] 倪润洲[2] 毛振彪[2] 

机构地区：[1]南通大学附属医院急诊内科,南通226001 [2]南通大学附属医院消化内科,南通226001 [3]南通大学附属医院病理科,南通226001

出　　处：《南通大学学报（医学版）》2015年第5期393-396,共4页Journal of Nantong University(Medical sciences)

基　　金：南通大学附属医院科技计划项目(Tdfy0906)

摘　　要：目的 :研究鸟苷酸环化酶C(guanylyl cyclase C,GC-C)和两个配体内生性肽类激素鸟苷素(guanylin,GN)和尿鸟苷素(uroguanylin,UGN)在胃癌、肠上皮化生和异型增生组织中的表达及与胃癌生物学行为之间的关系,并探讨其临床意义。方法:采用实时荧光定量聚合酶链反应法(real-time quantitative polymerase chain reaction,q RT-PCR)检测胃癌(60例)、肠上皮化生(23例)、异型增生(25例)和远癌胃(30例)组织中GC-C、GN和UGN m RNA的表达。进一步对三者与临床病理参数的关系及三者间的相关性进行分析。结果:q RT-PCR显示,GC-C和GN m RNA在肠上皮化生、异型增生及胃癌中高表达,而远癌胃组织中则不表达(均P=0.000 0)。GC-C和GN m RNA在肠型胃癌中的表达高于弥漫型(P=0.000 4和0.000 8),与年龄、性别、病灶大小、临床病理分期、分化程度、淋巴结转移和浸润深度等因素无关(均P>0.05)。在肠上皮化生、异型增生和胃癌组织中GC-C和GN的表达呈正相关(r=0.682 9、0.495 4和0.777 4,P=0.000 3、0.011 8和0.000 0)。而UGN在远癌胃组织、肠上皮化生、异型增生及胃癌组织中都有表达,差异无统计学意义(均P>0.05)。结论:胃黏膜癌变过程中GC-C的异位表达与其内生性配体GN的表达有关,检测两者的变化将有助于胃癌高危人群监测和胃癌早期诊断。Objective:To investigate the relationship of expressions of guanylyl cyclase C(GC-C) and its endogenous ligands [guanylin(GN) and uroguanylin(UGN)] in human gastric carcinoma, intestinal metaplasia or dysplasia and its significance. Methods:GC-C and its endogenous ligands(GN and UGN) expression was determined for mRNA by real-time quantitative polymerase chain reaction(qRT-PCR), from the patients in tissue including gastric carcinomas mucosa(60 specimens) and their corresponding distal normal gastric tissues (30 specimens), intestinal metaplasia (23 specimens) and displastia (25 specimens). The data from the experiment were analyzed statistically. Results:The expressions of GC-C mRNA and GN mRNA were absent in the distal normal gastric tissues in all cases, whereas, they were measured in intestinal metaplasia, dysplasia and gastric cancer(P=0.000 0). The expression of GC-C and GN were closely related to intestinal-type(Lauren classification)(P=0.000 4 and 0.000 8),but that showed no close relationship with age, sex, tumor size, the clinical stages, tumor differentiation degree nor the metastasis of lymph node(P>0.05). Also, the positive correlation was found between GC-C and GN in intestinal metaplasia, dysplasia and gastric cancer(r=0.682 9, 0.495 4 and 0.777 4, P=0.000 3, 0.011 8 and 0.000 0, respectively). However, UGN expression in gastric carcinomas, intestinal metaplasia, dysplasia and distal normal gastric tissues were no significant differences(P>0.05). Conclusion:Ectopic expression of GC-C correlates significantly with the endogenous ligand GN expression in gastric mucosa. Detection of GC-C and GN proteins will be beneficial to monitor the gastric cancer high-risk groups and diagnosis of early gastric cancer.

关 键 词：胃癌 癌前病变 鸟苷酸环化酶C 鸟苷素 尿鸟苷素 人 

分 类 号：R735.2[医药卫生—肿瘤]